Determination of Citrinin Using Molecularly Imprinted Solid Phase Extraction Purification, HPLC Separation, and Fluorescence Detection Michael Appell, Michael A. Jackson, Lijuan C. Wang & Wayne B. Bosma To cite this article: Michael Appell, Michael A. Jackson, Lijuan C. Wang & Wayne B. Bosma (2015) Determination of Citrinin Using Molecularly Imprinted Solid Phase Extraction Purification, HPLC S...

A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormone...

Oleanolic acid (OA) and ursolic acid (UA) are isomeric triterpenic acids and only one methyl's position is different between them. OA and UA always exist in the same plant, so it is difficult to separate them when determining contents by RP-HPLC. In this study, a very simple mobile phase for HPLC was developed to simultaneously determine UA and OA, and the factors affecting separation were also...

a special type of silica-based columns has been recently introduced into the market which is called narrow-bore columns. they have lower internal volume than the standard high-performance liquid chromatography (hplc) columns and thus reduce the solvent consumption by almost 80%.    a simple, accurate and environmentally friendly reversed phase- hplc (rp-hplc method) which could be used in fast ...

BACKGROUND In-hospital hypovitaminosis C is highly prevalent but almost completely unrecognized. Medical awareness of this potentially important disorder is hindered by the inability of most hospital laboratories to determine plasma vitamin C concentrations. The availability of a simple, reliable method for analyzing plasma vitamin C could increase opportunities for routine plasma vitamin C ana...

Separation of ten sedative- hypnotic drugs was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency (N) and asymmetry factor (B/A) showed that, the appropriate conditions for a good separation were 35°C and 7% (V/V) butanol in mobile phase.  Variations of selectivity factor versus butanol concent...

A simple, precise, and accurate isocratic reversed-phase (RP) stability-indicating HPLC assay method was developed and validated for determination of Aripiprazole in bulk and solid pharmaceutical dosage form. A reversed-phase C8 (250×4.0 mm, 5 μm particle size) column for HPLC and C8 (50×2.1mm, 1.7 μm particle size) for UPLC method in isocratic mode was used. The mobile phase consists of aceton...

HPLC is a separation technique in which the mobile phase is an aqueous solution or an organic solvent that is miscible with water, and the compounds of interest are dissolved in water or an organic solvent such as methanol. The major advantage of the technique is the flexibility it allows in choosing the stationary and mobile phases that best separate the particular sample components. More than...

oligosaccharide prepared from Pl.-B was eluted as a single peak on both reversed-phase {RP-) HPLC and size-fractionation (SF-) HPLC, and its strueture was estimated to be Manoct-qManal -. 3)(Xylfi1-.2) Manfi1-4GlcNAcfi1-4(Fuc=1-3)GlcNAc by a combination of component analysis, successive exogl)'cosidase digestions, IS-MS anal)'sis, and 500MHz 'H-NMR, Two tryptic gl)'copeptides were isolated from...

Topical gels containing extracts of Schinus terebinthifolius have been used to treat bacterial vaginosis. It has been reported that this species has antimicrobial, anti-inflammatory and anti-ulcerogenic properties, which can be attributed to the presence of phenolic compounds. In this work, a sensitive and selective reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols...