phenylketonuria (pku) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. the symptoms of this disease can be prevented if detected soon after birth. therefore, blood phenylalanine (phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of pku patients. the goal of this research was to introduce a r...

Many studies have been devoted to the inhibitory action of various metabolite analogues on microbial growth. In most of this work, however, such nonpathogens as the yeasts, lactobacilli, and Escherichia coli have served as the test organism and there has been little attempt to correlate or extend the studies to pathogenic bacteria, especially those organisms synthesizing their own growth factor...

Chlamydiaceae are obligate intracellular bacterial pathogens that strictly depend on host metabolites, such as nucleotides, lipids, and amino acids. Depletion of amino acids in cell culture media results in abnormal chlamydial development in vitro. Surprisingly, enrichment of certain amino acids also retards chlamydial growth. Our experiments revealed that the antichlamydial effects are largely...

Shigella toxin inhibits polyuridylic acid-directed polymerization of phenylalanine in ribosome-enzyme systems obtained from Escherichia coli or from Shigella dysenteriae. The inhibition is the result of toxin acting on ribosomes to prevent polyuridylic acid attachment.

Moderate growth inhibition was obtainable in several transplantable tumors by the feeding of fl-3-thienylalanine in diets lacking phenylalanine. The effects of fl-3-thienylalanine and deoxypyridoxine were additive in inhibition of the growth of Murphy-Strum lymphosareoma; this inhibition was further increased by testosterone, Halotestin, and Nilevar. Small amounts of phenylpyruvate used with sm...

The phosphorylation of human phenylalanine hydroxylase by cyclic AMP-dependent protein kinase was studied using recombinant enzyme expressed as a fusion protein in the pMAL system of Escherichia coli. Using the target sequence of the restriction protease enterokinase (Asp4-Lys) as the linker peptide, 100% full-length human phenylalanine hydroxylase was obtained on protease cleavage. The fusion ...