Octanoic acid inhibits, in vitro, the bacterial enzymes glucose-6-phosphate dehydrogenase, phosphofructokinase, pyruvate kinase, fumarase, lactate dehydrogenase, and the malic enzyme of Arthrobacter crystallopoietes. The free fatty acid appears to act as an inhibitor of lipogenesis, although it does not affect the rate of gluconeogenesis. To demonstrate that this inhibition may be of physiologi...

The chain oxidation of glyceraldehyde-3-phosphate dehydrogenase *NADH by perhydroxyl radicals and propagated by molecular oxygen was studied by the xanthine-xanthine oxidase system, ‘OCo y-ray, and pulse radiolysis. The chain length, amount of NADH oxidized per HOz generated, increases with increasing acidity of the medium and reaches a value of 73 at pH 5.0. The rate constant for the oxidati...

A gene encoding an S.pombe homologue of glycerol-3-phosphate dehydrogenase was isolated from a XEMBL3 library. The clone was originally isolated because it hybridised to a rat BiP cDNA probe (1). Upon sequencing, however, it was found that there was no homology between this clone and BiP at the amino acid level. The open reading frame was used to search databases and the gene identified as an S...

A mineral phosphate solubilizing bacterium, Burkholderia cepacia DA23 has been isolated from cultivated soils. Phosphate-solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. When 3% of glucose concentration was used for carbon source, the strain had a marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was ...

To evaluate the concept of metabolic cold adaptation in Antarctic fungi, we compared the activities of several key enzymes of the glycolytic pathway and the TCA cycle (hexokinase, glucose-6-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, succinate dehydrogenase, isocitrate dehydrogenase, and malate dehydrogenase) in psychrotolerant Penicillium sp. 161 and mesophilic Aspergill...

Objective: Uses molecular docking and pharmacophore modeling methods to examine the antimalarial activity of apigenin (API) on distinct kinds varieties P. falciparum (Pf) receptors. Methods: Using Autodock 4.0.1 ligandscout software, was conducted multiple types Pf receptors, including lactate dehydrogenase (Oxidoreductase), Enoyl-acyl carrier-protein Triose-phosphate (Isomerase), plasmepsin II...