The pathway for the biosynthesis of new cardiolipin was investigated in the isolated perfused intact rat heart. Isolated rat hearts were perfused in the Langendorff mode for up to 60 min with Krebs-Henseleit buffer containing 0.1 microM [U-14C]glycerol. Analysis of radioactivity incorporated into phospholipids in the organic phase revealed an increase in radioactivity incorporated into phosphat...

Liposomes which have entrapped the metallochromic dye, arsenazo III, constitute a sensitive assay system for ionophoresis of divalent cations. By this means we have compared known calcium ionophores (A23187, ionomycin) with membrane phospholipids, fatty acids, prostanoids, and retinoids. Added at micromolar concentrations to preformed multilamellar liposomes (phosphatidylcholine 7:dicetyl phosp...

The cornea has one of the highest acetylcholine (ACh) concentrations of any tissue but the function of the ACh has remained enigmatic. During studies on corneal arachidonic acid metabolism, we observed that ACh stimulates formation of labeled phosphatidic acid in rat corneas whose phospholipids were prelabeled with [14C]arachidonate. ACh did not affect the metabolism of free [14C]arachidonate. ...

Chibnall and Channon, investigating the cytoplasma of cabbage and spinach leaves (I), isolated a mixture of substances which resembled monophosphatides in their structure and composition, but mere virtually free of nitrogenous bases. The main constituents were identified as the calcium, magnesium, and potassium salts of phosphoric acid esters of diglycerides. These compounds, constituting a new...

Chibnall and Channon, investigating the cytoplasma of cabbage and spinach leaves (I), isolated a mixture of substances which resembled monophosphatides in their structure and composition, but mere virtually free of nitrogenous bases. The main constituents were identified as the calcium, magnesium, and potassium salts of phosphoric acid esters of diglycerides. These compounds, constituting a new...