Compounds that increase cellular cyclic AMP concentrations, such as isoprenaline, adrenaline and prostaglandin El, inhibit the release of histamine from sensitive tissues after antigen challenge (Assem & Schild, 1969; Lichtenstein & Margolis, 1968). The present paper describes experiments that show that I.C.I. compound 74917, Bufrolin (6-n-butyl-2,8-dicarboxy-4,10-dioxo-1,4,7,1O-tetrahydro-l,7-...

Billington, Charlotte K., Sunil K. Joseph, Caroline Swan, Mark G. H. Scott, Timothy M. Jobson, and Ian P. Hall. Modulation of human airway smooth muscle proliferation by type 3 phosphodiesterase inhibition. Am. J. Physiol. 276 (Lung Cell. Mol. Physiol. 20): L412–L419, 1999.— Elevation in cell cAMP content can inhibit mitogenic signaling in cultured human airway smooth muscle (HASM) cells. We st...

Clostridium thermocellum polynucleotide kinase-phosphatase (CthPnkp) catalyzes 5' and 3' end-healing reactions that prepare broken RNA termini for sealing by RNA ligase. The central phosphatase domain of CthPnkp belongs to the dinuclear metallophosphoesterase superfamily exemplified by bacteriophage lambda phosphatase (lambda-Pase). CthPnkp is a Ni(2+)/Mn(2+)-dependent phosphodiesterase-monoest...

Phosphatase enzymes regulate organic phosphorus (P) turnover in soil, but a clear understanding remains elusive. To investigate this, phosphomonoesterase and phosphodiesterase activities were determined by using para-nitrophenol (pNP) analogue substrates in a range of temperate pasture soils from England and Wales. Substrate-induced phosphatase activity ranged between 2.62 and 12.19 micromol pN...

Pharmacological analysis of in vivo cAMP phosphodiesterase in Xenopus oocytes using the nonselective enzyme inhibitors 3-isobutyl-1-methylxanthine (IBMX), theophylline, and papaverine, demonstrated inhibition of insulin- and insulin-like growth factor-1-induced maturation at concentrations that were 17-60-fold lower than those required to inhibit progesterone-induced germinal vesicle breakdown....