The family Magnaporthaceae considered as a single-family placed in the order Magnaporthales. Currently, three distinct clades are phylogenetically distinguished in this order that one is referred as the Pyriculariaceae. The Pyriculariaceae is characterized as a novel family having new genera, new species, new combinations and neotypification (basionym). To taxonomically evaluate this family, an...

Title of Dissertation: DINOFLAGELLATE GENOMIC ORGANIZATION AND PHYLOGENETIC MARKER DISCOVERY UTILIZING DEEP SEQUENCING DATA Gregory Scott Mendez, Doctor of Philosophy, 2016 Dissertation directed by: Professor Charles F. Delwiche, Cell Biology and Molecular Genetics Dinoflagellates possess large genomes in which most genes are present in many copies. This has made studies of their genomic organi...

Single nucleotide polymorphisms (SNPs) involved in the development of resistance represent powerful markers for the rapid detection of first- and second-line resistance in clinical Mycobacterium tuberculosis complex (MTBC) isolates. However, the association between particular mutations and phenotypic resistance is not always clear-cut, and phylogenetic SNPs have been misclassified as resistance...

Internal Transcribed Spacer (ITS) is one of the most used barcoding regions for molecular phylogenetics and orchids. Our aim in this study to test reliability ITS on closely related Neotinea spp., including tridentata, ustulata subsp. aestivalis, by comparing it accD-psaI intergenic spacer plastid DNA. Both were amplified specific primer sets sequenced. Phylogenetic trees regenerated using Maxi...

AIMS The study goals were to determine the relationship between faecal indicator bacteria (FIB), the HF183 marker and land use, and the phylogenetic diversity of HF183 marker sequences in a tropical urban watershed. METHODS AND RESULTS Total coliforms, Escherichia coli, and HF183 were quantified in 81 samples categorized as undeveloped, residential and horticultural from the Kranji Reservoir ...

The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Abstract Aims: The study goals were to determine the relationship between faecal indicator bacteria (FIB), the HF183 marker and land use, and the phylogenetic diversity of HF183 marker sequences in a tropical urban watershed. Methods and Results: Total coliforms, Escherichia co...

Constructing clone libraries from phylogenetic marker genes is currently the most widely used means of assessing microbial community composition and diversity. Although more expensive and time-consuming than community fingerprinting techniques, sequence analysis of clone libraries provides an unparalleled level of phylogenetic resolution due to the long read lengths generated by Sanger sequenci...