Surface-bound alpha-fetoprotein (AFP) was demonstrated by immunofluorescence on approximately 1/3 of splenic lymphocytes in chronic murine graft vs. host (GVH) reactions. Splenic lymphocytes were also shown to have a suppressed phytohemagglutinin (PHA) response compared to controls while lymph node cells from the same GVH animals revealed no surface AFP and had normal PHA responses. Splenic lym...

We prepared in vitro an mRNA transcript coding for the erythroagglutinating subunit of the kidney bean glycoprotein phytohemagglutinin, E-PHA. The mRNA, injected into Xenopus oocytes, synthesized E-PHA carrying two Asn-linked carbohydrate chains, one of which was processed and acquired resistance to endo-beta-N-acetylglucosaminidase H, as occurs in the native bean cells. When the mannose analog...

We examined the effect of phytohemagglutinin (PHA) on glucocorticoid receptors in lymphocytes, using peripheral mononuclear leukocytes from patients with connective tissue diseases. Glucocorticoid receptor concentrations and dissociation constants (Kd) for [3H]dexamethasone binding to lymphocytes from patients with rheumatoid arthritis, Sjögren's syndrome, and other connective tissue diseases d...

A serum factor (SF), isolated from acidified sera of normal donors, inhibited phytohemagglutinin (PHA) stimulation of lymphocytes isolated from patients with chronic lymphocytic leukemia (CLL),but had no effect on those from healthy donors. An SF isolated by an identical procedure from the sera of patients with CLL had essentially no inhibitory effect on DNA synthesis in normal or leukemic lymp...

Lymph node cells of ovalbumin-sensitized rats were separated on the basis of buoyant density into fractions reciprocally enriched in cells responsive to ovalbumin or phytohemagglutinin (PHA). Recombination of high density and low density fractions in varying proportions resulted in potentiation or suppression of the DNA synthetic response to PHA in culture. The response of cultures containing e...

The effects of phytohemagglutinin (PHA) were studied in irradiated mice to see if a definite myeloproliferative effect could be demonstrated in vivo. The data obtained suggested the following conclusions. PHA treatment of the bone marrow donor only, causes a consistent but slight reduction in transplantable spleen colony-forming unit (CFU) content of the bone marrow 24 hr after the last PHA inj...

Peripheral blood lymphocytes from normal donors and patients with chronic lymphocytic leukemia. B-cell type. were purified into T. helper T. and suppressor T lymphocytes by fluorescence-activated cell sorting using OKT3. 0KT4. and 0KT8 monoclonal antibodies. The maximum response of the purified subpopulations to stimulation by phytohemagglutinin (PHA) was determined by measuring the production ...

A Hodgkin cell-specific antigen detected by the monoclonal antibody Ki-1 was found on T helper lymphocytes after activation by autologous and allogeneic stimulator cells. About 50% of lymphoblasts generated by autoand alloactivation reacted with the antibody. In contrast. only less than 6% of lymphoblasts stimulated with Con-A. phytohemagglutinin (PHA). or protein A. and none of lymphoblasts

Expression of terminal transferase (TdT) is believed to be restricted to primitive lymphoid cells; recently, however, indirect immunofluorescent (IF) assays have been used to demonstrate the apparent presence of TdT in phytohemagglutinin (PHA)-stimulated peripheral blood lymphocytes and in various nonlymphoid malignancies. Using an IF assay, we found that a heteroantiserum to TdT reacted with c...

Peripheral lymphocytes were cultured in the presence and absence of phytohemagglutinin (PHA). Porphyrin synthesis was visually estimated by fluorescence and quantitated by fluorometry. No visual fluorescence was noted in cells cultured with or without PHA. Addition of #{246}-aminolevulinic acid (ALA) to cultures of either transformed or nontransformed lymphocytes resulted in fluorescence, indic...