Conservation and propagation of species using biotechnologic tools-such as plant tissue culture-are relevant when natural propagation is hampered for various reasons. In vitro techniques allow mass multiplication and propagation under pathogen-free conditions but also override dependence on season for availability of plant material. Moreover, in vitro genetic manipulation of a species, invariab...

An efficient in vitro propagation system for an elite clone EC-353508 of Artemisia annua L., an important antimalarial drug plant has been developed. The best organogenic response, including adventitious multiple shoots and their elongation, was obtained when nodal explants were cultured on MS medium supplemented with 10μM 2-isopentenyl adenine (2iP). An average of 17.7±0.88 shoots with an aver...

Striking and pronounced growth responses to the gibberellins as plant regulators and hormones are well known and readily produced in suitable plants. It was our purpose to study the effects of such growth responses on the concentrations of plant lipids and on the concentrations of antioxidants that are synthesized in plants. Growth changes were induced in pea plants by the application of gibber...

Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl₂), and silver thiosulphate (STS). The addition of AVG, CoCl₂, and STS signif...

In vitro production of camptothecin (CPT) was achieved by the establishment of multiple shoot and root cultures of Ophiorrhiza rugosa Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal. The maximum multiple shoots were obtained in Murashige and Skoog (MS) medium supplemented with 5mg/l N-benzyl amino purine (BA) along with 0.5mg/l naphthalene acetic acid (NAA). Optimal root generation was obser...

An in vitro protocol has been established for clonal propagation of Nothapodytes nimmoniana which is an important source of Camptothecin (CPT). Elite source was identified based on the chemical potency to accumulate the optimum level of CPT. Different types and concentrations of plant growth regulators were used to study their effect on inducing multiple shoots from the explants regenerated fro...

Plants can remove pollutants through direct absorption and by providing habitats for microbes to stimulate their activities. The aboveground plant biomass is usually harvested to remove pollutants absorbed in plant tissues. However, the effect of plant harvesting during summer on the performance of constructed wetlands and microbial abundance is unclear. In this study, three types of microcosms...

the present work developed an efficient plant regeneration and propagation system via direct organogenesis for morinda officinalis how., a valuable endangered medicinal plant. the effects of explant types on shoot induction, plant growth regulators on shoot proliferationand elongation and the subsequent rooting ability of shoots were examined. among the various types of explant, direct shoot pr...

The effects of sugar concentration and strength of basal medium were studied to produce plants from somatic embryos in Asparagus of®cinalis L. There was a signi®cant difference among concentrations of sugar but not among kinds of sugar tested in the present experiment in growth of shoots and roots. When the sucrose concentrations were 10, 30, or 50 g lÿ1, the fresh weight of shoots were 31.5, 1...

Transgenic plants harboring the left transfer DNA (T(L)-DNA) of the root inducing plasmid of Agrobacterium rhizogenes show many developmental abnormalities. We observed frequent appearance of normal looking lateral (revertant) shoots from such aberrant plants. Unlike aberrant shoots of the plant, revertant shoots exhibited a very high growth rate and set viable seeds. Sexual and vegetative repr...