Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize within the target sequence, was introduced into NASBA reactions that amplify the genomic RNA of potato leafroll virus (PLRV). During amplification, the probe anneals to the antisense RNA amplicon genera...

Microarrays are one of the new emerging methods in plant virology currently being developed by various laboratories. In this study, a new approach is described on the detection of plant viruses using short synthetic single-stranded oligomers (40 nt) instead of PCR products as capture probes. A microchip detecting potato viruses, PVA, PVS, PVM, PVX, PVY and PLRV, in both single and mixed infecti...

Persistent circulative transmission of plant viruses involves complex interactions between the transmitted virus and its insect vector. Several studies have shown that insect vector proteins are involved in the passage and the transmission of the virus. Interestingly, proteins expressed by bacterial endosymbionts that reside in the insect vector, were also shown to influence the transmission of...

Alvarez, A.E. (2007), Resistance mechanisms of Solanum species to Myzus persicae, Ph.D. thesis, Wageningen University, Wageningen, the Netherlands. The aphid Myzus persicae (Sulzer) constitute a threat to potato crops because of their efficiency to transmit viruses. Many wild Solanum species have been reported to have resistance to M. persicae. These species represent an important potential sou...

Purified BLRV RNA was converted to cDNA and cloned into pT7T3 19U phagemid. The nucleotide sequence of a putative bean leafroll luteovirus coat protein gene was determined from the clones pBL9, pBL29 and pBL32 by the chain termination method (1). Sequence analysis reveals a 588 bp open reading frame and an internal 426 bp ORF (Fig. 1). The large ORF is equivalent to a protein of Mr 21.98 kDa, t...

In the continental temperate climatic area virotical infection of potato was conditioned by the sources of virotical infection and by then viroses vectors. In Huedin micro area, situated intermountain in Cluj County at 600 m altitude, which humid and cool climate and isolated in space in natural way. Were tested 4 types of potato varieties sensitive or resistant at the main viruses of potato: P...