In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The...

The production of ethylene by etiolated pea epicotyls (Pisum sativum L., cv. Alaska) is confined to the plumule and plumular hook portion of the epicotyl, and occurs at a rate of about 6 mul.kg(-1).hr(-1). Such a rate is sufficient to give physiologically active concentrations of ethylene within the tissue. Exposure of etiolated seedlings to a single dose of red light caused a transient decreas...

Acyl-CoA dehydrogenase activity has been measured in homogenates of post-imbibition to 14-day-old hydroponically grown pea seeds at daily intervals, using C(4), C(12) and C(16) acyl-CoA substrates. The activity peaks of the different chain-length acyl-CoA dehydrogenases did not transpose at all points and the ratios of the chain-length activities were not constant. It therefore has to be conclu...

In this work, a fast and efficient microwave-assisted extraction (MAE) method was developed to extract main bioactive alkaloids from lotus plumue. To optimize MAE conditions, three main factors were selected using univariate approach experiments, and then central composite design (CCD). The optimal extraction conditions were as follows: methanol concentration of 65%, microwave power of 200 W, a...

Plastid ribosomal proteins (PRPs) are essential for ribosome biogenesis, plastid protein biosynthesis, chloroplast differentiation, and early chloroplast development. This study identifies the first rice PRP mutant, asl1 (albino seedling lethality1), which exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered chlorophyll (Chl) content and c...

Seed priming is a technique which is potentially able to promote rapid and more uniform seed germination and plant growth as well. In this regards, the presowing effects of seed priming treatments were investigated on some physiological viz. seed germination, germination rate, radicle elongation, shoot elongation, radicle dry weight, shoot dry weight and vigor index. Seeds were subjected to two...

It was evaluated the damage caused by Bruchus pisorum on the germination ability of pea forage cultivars. Result of damage in seeds with parasitized larva was a significant decrease of the germination by 16.4% percentage points, the length and weight of primary radicle by 16.8 and 24.5%, the length and weight of plumule by 12.3 and 14.1%, the vigor index of primary radicle and plumule by 32.5 a...

We examined the distribution and the immunohistochemical localization of yieldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting analysis revealed that the yieldin was located in the aerial organs (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongation region (the hook r...