Microaerophilic microenvironment at biocathode was evaluated for electrogenesis along with the polyhydroxyalkanoates (PHA) accumulation in bio-electrochemical system (BES). The electrogenic activity (512 mV; 15.2 mW/m(2)) was extended for longer periods (144 h) which might be attributed to the lowering of losses due to the controlled microbial metabolism. Growth limiting stress at cathode due t...

Polyhydroxyalkanoates (PHAs) are synthesized and assembled as PHA granules that undergo well-regulated formation in many microorganisms. However, this regulation remains unclear in haloarchaea. In this study, we identified a PHA granule-associated regulator (PhaR) that negatively regulates the expression of both its own gene and the granule structural gene phaP in the same operon (phaRP) in Hal...

The current problems with decreasing fossile resources and increasing environmental pollution by petrochemical-based plastics have stimulated investigations to find biosynthetic materials which are also biodegradable. Bacterial reserve materials such as polyhydroxyalkanoates (PHA) have been discovered to possess thermoplastic properties and can be synthesized from renewable resources. Poly-beta...

BACKGROUND Achieving a sustainable society requires, among other things, the use of renewable feedstocks to replace chemicals obtained from petroleum-derived compounds. Crude glycerol synthesized inexpensively as a byproduct of biodiesel production is currently considered a waste product, which can potentially be converted into value-added compounds by bacterial fermentation. This study aimed a...

Synthesis of polyhydroxyalkanoates (PHAs) from intermediates of fatty acid beta-oxidation was used as a tool to study fatty acid degradation in developing seeds of Arabidopsis. Transgenic plants expressing a peroxisomal PHA synthase under the control of a napin promoter accumulated PHA in developing seeds to a final level of 0. 06 mg g(-1) dry weight. In plants co-expressing a plastidial acyl-a...

Polyhydroxyalkanoates (PHAs) are accumulated as intracellular carbon and energy storage polymers by various bacteria and a few haloarchaea. In this study, 28 strains belonging to 15 genera in the family Halobacteriaceae were investigated with respect to their ability to synthesize PHAs and the types of their PHA synthases. Fermentation results showed that 18 strains from 12 genera could synthes...

Palm oil industries have been contributing significantly towards the country’s economy and increase standard of living among Malaysians. However, it has also been identified as the major contributor for discharging the largest pollution load throughout the country. Owing to high biochemical oxygen demand (BOD) and chemical oxygen demand (COD), the palm oil mill effluent (POME) cannot be dischar...

The Azotobacter vinelandii is an aerobic, nitrogen-fixing bacterium with the ability to accumulate polyesters such as poly (3-hydroxybutyrate), a particular group of the polyhydroxyalkanoates (pha), when will be cultivated on several carbon sources, including sucrose. Although the capacity of Azotobacter strains to accumulate polyesters is well known, and the genes responsible for their synthes...

Polyhydroxyalkanoates (PHAs) have received considerable interest as renewable-resource-based, biodegradable, and biocompatible plastics with a wide range of potential applications. We have engineered the synthesis of PHA polymers composed of monomers ranging from 4 to 14 carbon atoms in either the cytosol or the peroxisome of Saccharomyces cerevisiae by harnessing intermediates of fatty acid me...

Polyhydroxyalkanoates (PHAs) have been demonstrated to be a family of biopolymers with good biodegradability and biocompatibility. To mimic the real microenvironment of extracellular matrix (ECM) for cell growth, novel nanofiber matrices based on PHA polymers were prepared via a phase separation process. Three-dimensional interconnected fibrous networks were observed in these matrices with aver...