conclusions based on our study, plca and hlya played a key role in the virulence determination of l. monocytogenes. data analysis also showed that l. monocytogenes could be a causative agent of abortion in pregnant women. results out of 96 samples, 16 isolates of l. monocytogenes by pcr (plca and hlya) and four isolates by culture were identified. there was a significant difference between pcr ...

results using these two sets, we identified h1069q mutation in four patients, c.2335t > g mutation in three, c.3061-1g > a splice site mutation in five, c.3305t > c mutation in one, and c.3809a > g mutation in two patients. conclusions the multiplex arms assay used in this study can be an efficient, reliable, and cost effective method as a primary screen for patients with wilson disease. patien...

abstract        background and objective: chabahar is in southern iran located near the iran-pakistan border. since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of leishmania parasite in the patients referred to the central laboratory.       methods: this descriptive cross-sectional study was conducted in 2011-2012 ...

background and objective: the outcome of hepatitis b virus (hbv) infection may be influenced by host factors like human leukocyte antigen (hla). we have investigated hla-a and drb1 alleles in patients with persistent hepatitis b infection compared to subjects who had spontaneously recovered from hbv infection. to complete the findings of this study we performed another survey in certain hla all...

background: toxoplasmosis is one of the most important food-borne diseases distributed worldwide. in order to determinate the involvement of poultry meat in clinical toxoplasmosis, this study aimed to genetic characterization of toxoplasma gondii in tissue samples of serologically positive chickens of tunisia using polymerase chain reaction (pcr). methods: trail of isolation of local t. gondii ...

background: cytochrome p450 2c19 (cyp2c19) is important in metabolism of wide range of drugs. cyp2c19*17 is a novel variant allele which increases gene transcription and therefore results in ultra-rapid metabolizer phenotype (urm). distribution of this variant allele has not been well studied worldwide. the aim of present study was to investigate allele and genotype frequencies of cyp2c19*17 in...

Polymerase chain reaction amplification of a locked nucleic acid (LNA)-modified DNA strand and transcription reactions using LNA-A nucleoside 5'-triphosphate were successfully accomplished with DNA and RNA polymerases.

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

background: acute infectious diarrhea is one of the most important causes of morbidity and mortality worldwide. objectives: the objective of this study was to characterize antimicrobial resistant diarrheagenic escherichia coli strains isolated from diarrheic children in tehran, ir iran. patients and methods: in total, 550 stool samples from diarrheic pediatric patients, aged less than 60 months...

background: human papillomaviruses (hpvs) are the most common viruses which can be sexually transmitted. they can cause different malignancies in asymptomatic women. the association of hpvs with infertility among men and women is controversial. in the current study, the authors compared the frequency of hpvs in fertile and infertile women in the city of mashhad. materials and methods: in the pr...