background brucellosis is the most common global zoonosis and an important public health problem in many parts of the world including iran. diagnosis of brucellosis is frequently difficult to establish and conventional methods are not always successful in identifying the organisms. rapid detection of brucella species by an automated blood culture system and polymerase chain reaction (pcr) may l...

background: some dynamic changes occurs during spermatogenesis such as histone removal and its replacement with transition nuclear protein and protamine. these proteins are required for packing and condensation of sperm chromatin. jhdm2a is a histone demethylase that directly binds to promoter regions of tnp1 and prm1 genes and controls their expression by removing h3k9 at their promoters.objec...

conclusions it is suggested that determination of the target genotype by plasma subtyping for choosing the proper antiviral therapy is essential but may result in therapy failure. hcv genotyping in pbmc samples, along with plasma specimens, might be more beneficial. therefore determining the hcv genotype in pbmcs, before beginning the therapy is useful due to the possibility of occult infection...

conclusions: nested pcr as a sensitive chlamydia trachomatis detection test and endocervical specimens has been offered to detect this bacterium in spontaneous abortion. besides, c. trachomatis screening among pregnant women can be suggested to prevent abortion. results: the number of intercourses per week and history of miscarriage can be known as the risk factors of abortion. frequency of c. ...

background and aims: helicobacter pylori is the primary cause of various diseases. this study was conducted to determine the existence of h. pylori in springs and surface water in chaharmahal and bakhtiari province. methods: in this study, 29 water samples were collected from three different locations of zayanderud river, 24 springs, and two subterranean from june 2014 to august 2014 in chaharm...

conclusions there was a significant correlation between crs and presence of h. pylori in sinonasal mucosa. this relationship may reflect the role of h. pylori as one of the pathogenic factors in the development of crs. however, further studies are required to confirm this role. results nine patients (18%) with crs had h. pylori isolated from their samples whereas in the control group, h. pylori...

background and objective: formalin-fixed paraffin-embedded tissues are a valuable source of dna for molecular studies. we designed and optimized an efficient procedure for dna extraction from formalin-fixed paraffin embedded tissues. materials and methods: seventy three blocks of cervical paraffin-embedded tissues were investigated. dna was extracted using 45 minutes boiling in alkaline solutio...

background: classical bacteriological detection of brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. however, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. methods: panel of some reference strains belonging to t...

background: one of the adulterant practice in dairy industry is the use of a less costly type of milk such as cow and goat milk instead of more expensive ones especially sheep milk. the aim of the present study was to assess fraud identification of undeclared milk species in composition of sheep yogurt and cheese offered as “pure sheep” in iranian local markets. methods: samples of sheep yogurt...