Polygalacturonase (PG) was purified from Erwinia carotovora EC. A hybrid cosmid, pSH711, that encodes PG activity but not pectate lyase activity was identified from an E. carotovora genomic library by an immunological screening method. A cell extract of Escherichia coli cells containing pSH711 was able to produce plant tissue maceration when spotted on carrot, potato, or turnip slices. In addit...

Bacterial ring rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causative agent of bacterial ring rot of potato. The results showed that the optimum extraction conditions of antimicrobial substances fro...

Plant defense responses to pathogen infection involve the production of active oxygen species, including hydrogen peroxide (H2O2). We obtained transgenic potato plants expressing a fungal gene encoding glucose oxidase, which generates H2O2 when glucose is oxidized. H2O2 levels were elevated in both leaf and tuber tissues of these plants. Transgenic potato tubers exhibited strong resistance to a...

In April 2002 and 2003, soft rot on fruit of eggplant (Solanum melongena) caused by Choanephora cucurbitarum was observed in the experimental fields at Gyeongnam Agricultural Research and Extension Services in Korea. The disease began with water-soaking and dark-green lesions, and then the infected tissues were rapidly rotten. Sporangium was subglobose in shape and sized 40~130 µm. Monosporous ...

Potato is one of the main crops which is suffering from the great losses in storage and the most conventional method of its tuber rot is using hazardous chemicals. Using antimicrobial potential of lichens can be one of the safe, green, environment friendly methods for plant diseases management. In this study, antimicrobial activity of acetone, methanol, chloroform extracts of Ramalina capit...

pectobacterium carotovorum is one of the most important bacterial pathogens of potato in the world.the expression of numerous genes including those involved in the production of virulence determinants in this bacterium are regulated by quorum sensing (qs). the signal molecules involved in qs in p. carotovorum belong to the group of n-acyl homoserine lactones (ahls). recently, several soil bacte...

feijoa as a fruit crop and ornamental tree is cultivated in the subtropics and warm temperate areas of the world including brazil, united states, new zealand and japan. a new disease with brown rot symptom on fruit occurred in feijoa orchards of northern iran in which it has become popular as a fruit crop. the objective of the present study was isolation and identification of the causal pathoge...

Macrophomina phaseolina (Tassi) Goid., a pycnidia-producing fungus, is one of the most important soilborne pathogens, infecting over 500 plant species in more than 100 plant families around the world (1,18). It causes a major disease of soybean (Glycine max (L.) Merr.) called charcoal rot (18). Symptoms on soybean include blight of emerging seedlings, wilting, early maturation, and incomplete p...

Several species of the genus Fusarium can cause apple fruit to rot while stored. Since Fusarium taxonomy is very complex and has constantly been revised and updated over the last years, the aim of this study was to identify Fusarium species from rotten apples, based on combined morphological characteristics and molecular data. We identified 32 Fusarium isolates from rotten apple fruit of cultiv...

Pectobacterium carotovurum subsp. brasiliense causes soft rot and blackleg diseases on potato. Here, we report the draft genome sequences of three weakly virulent P. carotovurum subsp. brasiliense strains isolated in Canada. Analysis of these genome sequences will help to pinpoint differences in virulence among P. carotovurum subsp. brasiliense strains from tropical/subtropical and temperate re...