background fish cell lines are advantageous alternatives to mammalian cell lines for carrying out in vitro research. they are used extensively for the study of fish biology, physiology and toxicology, and more recently for germplasm conservation of important fish species. objectives the present study aims to establish and characterize a novel fish cell line from muscle tissue of wallago attu ca...

primary sjögren's syndrome (pss) is a common autoimmune disease involving abnormal th17 activation. the aim of the current study was to investigate the immunosuppression effect of cyclosporine a (cys a), a potent immunosuppressor on the proliferation and activation of t cells, on the activation of th17 cells. blood samples from both inactive and active pss patients as well as healthy controls w...

Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic and varicose vein leg ulcers. Therefore, we aimed at developing a fibroblast bank to acco...

The conditions for growing preneoplastic mammary epithelial cells in primary cell cultures with serum-free factor-defined medium were established in a series of experiments. The cells, grown in Dulbecco's modified Eagle's medium with 0.6 mM Ca2+, epidermal growth factor, transferrin, insulin, and Na2SeO3, were fibroblast free, exhibited saturation densities similar to those of cells grown in se...

A method is described for subdividing S phase cells in asynchronous cell cultures on the basis of replication band patterns produced in chromosomes by bromodeoxyuridine incorporation. The criteria used for cell classification are objective, requiring the presence or absence of specific bands on particular chromosomes, and therefore lead to subdivisions amenable to quantitative analysis and for ...

statement of problem: many studies have indicated that genetic disturbances are common findings in patients with oral squamous cell carcinoma (oscc). identification of these changes can be helpful in diagnostic procedures of these tumors. purpose: the aim of this study was to appraise the chromosomal disorders in blood and tissue patients with oscc. methods and materials: in this descriptive st...