Human neutrophils preloaded with chlortetracycline, commonly used as a probe of membrane-bound calcium, demonstrate a prompt decrease in fluorescence when exposed to surface stimuli such as the chemotactic peptide fMet-Leu-Phe. The fluorescence response was highly sensitive to preincubation with prostaglandin E1. This effect was apparently not due to elevated levels of cAMP since exogenous dibu...

In human platelets, prostaglandin E1 and forskolin inhibit 5-hydroxytryptamine-induced phospholipase C, C-kinase and myosin light-chain kinase activity in a concentration-dependent way. Phospholipase C activation, however, was only partly inhibited, and this at higher concentrations than the protein kinases. Direct activation of the C kinase either by exogenous synthetic diacylglycerol or by 12...

Concentration-related dye leakage produced by intracutaneous injections of irritants was measured in rats by an Evans blue technique. 2 In rats pretreated with a total dose of 50 mg capasaicin over 4 days, the response to capsaicin, formalin, HCl, KCl, prostaglandin E1, bradykinin and bradykinin with prostglandin E1 (10(-6) M) were greatly reduced, the responses to histamine and 5-hydroxytrypta...

Objective- To studylocal effect of prostaglandin E1on sciatic nerve regeneration Design- Experimental study Animals- Sixty male healthy white Wistar rats Procedures- Sixty animals were divided into four experimental groups (n = 15), randomly: Trasnsected (TC), Sham-operation (SHAM), control (CHIT) and prostagl...

Prostaglandins E1 and E2 increased the sensitivity of glycolysis to insulin in the isolated stripped soleus muscle of the rat, but prostaglandin F2 alpha had no effect. Indomethacin, which inhibits prostaglandin formation, markedly decreased the sensitivity of glycolysis to insulin. These findings suggest that prostaglandins of the E series increase the sensitivity of muscle glycolysis to insul...

We have examined the roles of three circumventricular organs, the area postrema, the subfornical organ, and the organum vasculosum of the lamina terminalis (OVLT), as possible access points for circulating pyrogens to cause fever. In conscious, unrestrained rats prepared with telemetry devices, intracerebroventricular cannulas, and intravenous catheters, body temperature was monitored after int...

It is suggested that COVID-19 (coronavirus) and other enveloped viruses can be inactivated by bioactive lipids. Bioactive lipids (BALs) AA, gamma-linolenic acid (GLA), dihomo-GLA (DGLA), eicosapentaenoic (EPA), docosahexaenoic (DHA) are precursors to anti-inflammatory metabolites prostaglandin E1, lipoxin A4, resolvins, protectins maresins enhance phagocytosis of macrophages leukocytes clear de...