Background: The objective of this study was to evaluate the relationship between protamine deficiency, and acrosomal integrity with fertilization and pregnancy rate in patients undergone in vitro fertilization (IVF). Material &Methods: Semen samples from 70 infertile couples undergoing IVF at Isfahan Fertility and Infertility center were assessed in this study. Semen analysis was carried out ac...

introduction: the aim of this study was to evaluate the effect of sperm chromatin anomalies on fertilization outcome post icsi. material and methods: therefore along with semen parameters, cma3 staining for protamine deficiency, aniline blue staining for excessive histones, sds for sperm chromatin stability and sds+edta for ability of sperm to undergo decondensation were carried out on 55 semen...

different methods have been used to evaluate the beneficial effect of varicocelectomy; these include semen parameters and pregnancy rate. due to high biological variability of semen parameters, sperm functional tests have been considered as an efficient end point in assessment of fertility. therefore, the aim of this study was to evaluate the effect of varicocelectomy on semen parameters and sp...

introduction: to evaluate and compare efficiency of sil-select with percoll to recover sperm with normal chromatin in morphology and also studyeffect of these parameters on fertilization, embryo quality and cleavagescore. materials and methods: assessment of semen parameters, protamine deficiency (cma3) and excessive histone (aniline blue staining) were carried out before and after sperm proces...

Background: Globozoospermia is an uncommon disorder of sperm morphology associated with severe male infertility. Patients who contain both normal and round-headed sperm are called partial globozoospermic. It is possible that round-headed sperm may have an abnormal chromatin structure and /or DNA strand breaks. Moreover, sperm DNA fragmentation and protamine deficiency is shown to be associated ...

BACKGROUND Sperm membrane integrity is not only important as a barrier between intra- and extra-cellular spaces, but also it can be considered as a sign of DNA integrity. Hypoosmotic swelling test reflects membrane integrity and has been used to evaluate sperm quality. Intracytoplasmic sperm injection (ICSI) in adjunct with hypo-osmotic swelling test (HOST) has been used for treatment of males ...

Semen samples collected from 28 male partners of infertile couples were divided into three equal aliquots and prepared with three selected media, such as PureSperm (Nidacon, Gothenburg, Sweden), Sil-Select Plus (Fertipro, Beernem, Belgium) and SpermGrad (Vitrolife, Gothenburg, Sweden). The differences in mean percentages of semen parameters were assessed by repeated measures analysis. Correlati...

introduction: different methods have been used to evaluate the beneficial effect of varicocelectomy; these include semen parameters and pregnancy rate. due to high biological variability of semen parameters, sperm functional test has been considered an efficient end point in assessment of fertility. therefore, the aim of this study was to evaluate the effect of varicocelectomy on semen paramete...

Again, our emphatic statements about the NO pathway and endothelium function may have led to this interpretation. We agree that cardiac surgery vasoplegia has multiple mechanisms, including drug interactions, as Poullis4 discussed in his excellent letter, which was motivated by the article of Argenziano et al5 on vasodilatory shock after cardiac surgery. Their third comment rejects the notion t...