MMPs have significant role in pathological processes and are associated with cancer invasion, and metastasis, cartilage destruction in arthritis. Non-synonymous single nucleotide polymorphisms (nsSNPs) occurring in protein coding regions of MMP potentially affect their function, and results in diseases. In this study we performed an insilco analysis of SNPs associated with MMP2 genes. Total of ...

Human body consists of lot of cells, each cell consist of DeOxaRibo Nucleic Acid (DNA). Identifying the genes from the DNA sequences is a very difficult task. But identifying the coding regions is more complex task compared to the former. Identifying the protein which occupy little place in genes is a really challenging issue. For understating the genes coding region analysis plays an important...

The Pô River basin of northern Italy is the home of distinctive and endemic morphological forms of brown trout Salmo trutta. We used PCR-direct sequencing and RFLP techniques to study variation in the mitochondrial control region of 225 trout in order to assess genetic relatedness among 18 populations from that region. The distribution analysis of these genotypes among north Italian populations...

OBJECTIVE Although the molecular mechanism of obesity has been poorly understood, recent studies indicate that leptin plays a critical role in regulating both food intake and body weight. Because obesity decreases the sensitivity to insulin, the human ob gene is presumed to be one of the candidate genes for non-insulin-dependent diabetes mellitus (NIDDM) associated with obesity. Although the pr...

The nucleotide sequence of a 3120 bp region of the E. coli chromosome that includes the entire ptr gene has been determined. The proposed coding region for Protease III is 2889 nucleotides long, which would encode a protein consisting of 962 amino acids with a calculated molecular mass of 107,719 daltons. The predicted primary structure of the protein includes a 23-residue signal sequence, clea...

The complete nucleotide sequence of the mitochondrial genome (mitogenome) of Aspiorhynchus laticeps was determined. The length of the complete mitochondrial DNA sequence of A. laticeps is 16,591 bp, which consists of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a non-coding region 'D-loop'. Except for the D-loop, another non-coding region named repl...

antisense oligodeoxynucleotides (oligos) are the tools that bind to complemen¬tary sequence of targeted mrna and block specifically protein translation. in the present study, a novel 20 mer oligo as an antisense for human il-13 is introduced. this oligo is designed according to the il-13 mrna coding region and synthesized in two hplc purified and fitc conjugated forms. fluorescence oligo cell u...

In V(D)J joining of antigen receptor genes, two recombination signal sequences (RSSs), 12- and 23-RSSs, form a complex with the protein products of recombination activating genes, RAG1 and RAG2. DNaseI footprinting demonstrates that the interaction of RAG proteins with substrate RSS DNA is not just limited to the signal region but involves the coding sequence as well. Joining mutants of RAG1 an...

A novel wavelet transform coding method based on the classiication of diierent wavelet amplitude regions is proposed. By using a low-complexity but eecient prediction model, we can distinguish diierent wavelet amplitude regions at upper subbands. Therefore, adaptive quantizers can be applied to diierent regions to achieve better reconstruction quality. The the main advantage of this algorithm i...