background: stress is a cause of male infertility. although sex hormones and sperm quality have been shown to be low in stress, sperm physiology and testicular functional proteins, such as phosphotyrosine proteins, have not been documented. objective: to investigate the acrosome status and alterations of testicular proteins involved in spermatogenesis and testosterone synthesis in chronic stres...

Abstract Extracellular matrix protein-1 (ECM1) promotes tumorigenesis in multiple organs but the mechanisms associated to ECM1 isoform subtypes have yet be clarified. We report this study that secretory ECM1a induces through GPR motif binding integrin ?X?2 and activation of AKT/FAK/Rho/cytoskeleton signaling. The ATP cassette subfamily G member 1 (ABCG1) transduces ECM1a-integrin interactive si...

Protein kinases play central role in cellular regulation by catalyzing the phosphorylation of target proteins involved complex physiological processes, achieved their structural flexibility switching between active state and inactive states. Quantitative characterization kinase conformational dynamics is important yet challenging. Nanopore analysis emerging as an attractive single-molecule tool...

The molecular mechanisms generating the mania-like abnormal behaviors caused by diacylglycerol (DG) kinase (DGK) η deficiency remain unclear. Here, we found that DGKη knockout markedly increased dopamine (DA) levels in midbrain (DA-producing region, 2.8-fold) and cerebral cortex (DA projection 1.2-fold). Moreover, significantly augmented phosphorylated DA transporter (DAT) (1.4-fold increase), ...

ige-mediated cell signaling, induced by cross-linking of high affinity receptor for ige (fcεri) in the presence of antigen (ag), is a well known mechanism described for mast cell activation in allergy and hypersensitivity reactions, which induces a spectrum of cellular responses such as secretion and up-regulation of cell surface fcεri. although for several years  ige  binding  to  fcεri  was  ...

Phosphorus (P) deficiency is a major threat to the crop production, and for understanding response mechanism of plant roots, P stress may facilitate development crops with increased tolerance. Phosphorylation plays critical role in regulation proteins responses biotic abiotic stress; however, its functions starvation/resupply are largely unknown barley ( Hordeum vulgare ) growth. Here, we perfo...