Every year lots of black tea (Camellia sinensis (L.) Kuntze) residue will produce in the factories. These residue are unusable whereas the bio active compounds can be extracted and used in the drag and food industries. Due to mentioned problems, this project was conducted years 2011 - 2012 with the aim to make a study on consecutive isolation of all bio active compounds from tea residu...

Many different software tools are available publicly to scan the PROSITE database of protein families. However, none of them, to our knowledge, wholly implements the PROSITE syntax, or satisfies all the rules for scanning a pattern against a sequence. We hereby propose a strict definition of how a PROSITE pattern is to be scanned against a sequence, and provide a reference implementation of a t...

expression of eukaryotic proteins in e. coli often results in their aggregation. proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. this is not achieved in cytoplasmic expression in e. coli because of the absence of disulfide bonds formation. a novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

conclusions the results of this study demonstrated that the tpasp-padre-truncated orf2 gene cassette and the truncated orf2 gene in recombinant plasmids are successfully expressed in eukaryotic cells. the immunogenicity of the two recombinant plasmids with different formulations will be evaluated as a novel dna vaccine in future investigations. results sequence analysis and bioinformatics studi...

trichophyton rubrum (t. rubrum) is an anthropophilic dermatophyte that is distributed worldwide and causes common cutaneous disease such as mycosis. although several properties of this fungus have been investigated so far, however a few studies were carried out in the field of molecular biology of this fungus. in the present study we tried to identify its molecular characterization of the goano...

background: malassezia are dimorphic, lipid-dependent yeasts, which are responsible for causing several cutaneous and sys­temic conditions. although cyclophilins (cyps) are highly conserved cytosolic proteins that catalyze the peptidyl-prolyl cis-trans isomerazation reaction before protein folding process, it has been suggestive of an allergen in a few numbers of fungi such as aspergillus fumig...

cytochrome-c (cyt-c) is an electron transport protein, and it is present throughout the evolution. more than 280 sequences have been reported in the protein sequence database (www.uniprot.org). though sequentially diverse, cyt-c has essentially retained its tertiary structure or fold. thus a vast data set of varied sequences with retention of similar structure and function makes it a primary ca...

SARS-nCoV was identified as corona virus had spread worldwide very quickly and affected more than million people worldwide. To halt this acceleration for efficient control the knowledge on genomic information is of utmost importance. We attempted to determine nature variation i.e., insertion, deletion, substitution, among structural sequences required code membrane, spike, nucleocapsid, envelop...