Iron (Fe) is required by plants for basic redox reactions in photosynthesis and respiration, and for many other key enzymatic reactions in biological processes. Fe homeostatic mechanisms have evolved in plants to enable the uptake and sequestration of Fe in cells. To elucidate the network of proteins that regulate Fe homeostasis and transport, we optimized the iTRAQ-OFFGEL method to identify an...

The effect of riboflavin deficiency on in vivo incorporation of [1-14C] leucine into proteins of liver has been studied on rats following protein depletion and subsequent repletion. The increase in the incorporation of [14C] amino acid into liver proteins follow ing protein depletion was less marked in riboflavin-deficient rats. But repletion with 40% protein demonstrated marked diminished prot...

The authors report the case of a 9-year-old Caucasian girl, born in northern Portugal, with chronic nonspherocytic haemolytic anaemia and without family history of anaemia. The aethiological study of this anaemia revealed pyruvate kinase deficiency (PKD), because of two previously described mutations (426Arg-->Trp and 510Arg-->Gln). Since the blood smear revealed features not fully compatible w...

Background: The manipulation of mineral nutrition may affect secondary metabolite production and thus determine the nutritional value food/medicinal plants. Objectives: present study investigated impact nitrogen supply on metabolites physiological parameters Hypericum androsaemum plants grown in sand culture. Methods: Four-week were irrigated by full-strength Hoagland solution (control), nitrat...

introduction: the main inhibitors of coagulation pathway are antithrombin (at), protein c and protein s. these inhibitors are necessary to prevent thromboembolism. hereditary deficiency of inhibitors is the main cause of alteration in balance between the anti-clotting and the formation of thrombin. patients with this abnormality are susceptible to venous thromboembolism (vte). two major clinica...

Deficiency in either of the breast cancer susceptibility proteins BRCA1 or BRCA2 induces profound cellular sensitivity to the inhibition of poly(ADP-ribose) polymerase (PARP) activity. We hypothesized that the critical role of BRCA1 and BRCA2 in the repair of double-strand breaks by homologous recombination (HR) was the underlying reason for this sensitivity. Here, we examine the effects of def...

The aim of this study was to the molecular mechanisms metabolic disorders that develop in highly productive cows. It found blood serum animals with subclinical ketosis (SCK), which are eighth month pregnancy, concentration β-hydroxybutyrate is increased, content iron, calcium and phosphorus reduced, its iron-binding capacity against background absence phenomena hepatocellular insufficiency, vio...

Macro and micronutrients are vital for the growth productivity of plants. Zinc (Zn) is considered to be one essential development cereals as well fodder crops. It also a regulatory cofactor all those enzymes which required synthesis chlorophyll, proteins carbohydrates. The functioning these affected significantly due Zn deficiency there will retarded Deficiency universal problem among cereal co...