Renanthera imschootiana Rolfe is an extremely rare and endangered tropical epiphytic orchid. Studies on in vitro culture of R. were conducted order to conserve increase the genetic pool this wild orchid species. In study, effect plant growth regulators (BA NAA), ripe bananas combination with potato, humic acid, silver nanoparticles (AgNPs) investigated find a suitable condition for regeneration...

Because orchid mycorrhizal fungi are important for seed germination, addition of germination-supporting fungal inoculum to the soil can be an efficient way improve in situ germination terrestrial orchids, but empirical evidence is still largely lacking. In this study, we used inoculation experiments test hypothesis that increases Gymnadenia conopsea under natural field conditions. China, specie...

BACKGROUND AND AIMS Many Orchidaceous species are threatened globally by development and over-collection from their natural habitats for horticultural purposes. Artificial propagation from seeds is difficult in most terrestrial orchids native to temperate regions. Seed production is another limiting factor in the artificial propagation for these species because of the lessened probability of po...

Thidiazuron (N-phenyl-N'-1,2,3-thiadiazol-5-ylurea; TDZ) is an artificial plant growth regulator that is widely used in plant tissue culture. Protocorm-like bodies (PLBs) induced by TDZ serve as an efficient and rapid in vitro regeneration system in Rosa species. Despite this, the mechanism of PLB induction remains relatively unclear. TDZ, which can affect the level of endogenous auxins and cyt...

The purpose of this study was to investigate the effect of the addition of different organic additives and sugar types as carbon source on the growth and proliferation of protocorm-like bodies (PLBs) of a very new Dendrobium hybrid known as D. Alya Pink (DAP). PLBs were supplemented with homogenates of banana, tomato, and coconut water at various concentrations. Proliferation rate was recorded ...