The present work aimed to apply mutagenesis and inter-specific protoplast fusion techniques of two locally isolated Hypocrea and Trichoderma to enhancement their biocontrol abilities against some important plant fungal pathogens which cause damping-off diseases that attacking different crops. The mutants were selected after EMS/UV treatment of Trichoderma isolates. The obtained protoplasts were...

Protoplasts, Soybean Cell Suspension Cultures, Phytoalexin Production, Phytophthora megasperm a f. sp. glycinea, Glucan Elicitor Protoplasts isolated enzymatically from suspension-cultured cells of soybean (Glycine max L. Merr., cv. Harosoy 63) were used to study the production of the isoflavonoid-derived phytoale­ xin, glyceollin. A large enhancement in the in vivo rates of synthesis and catal...

for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...

We have analyzed the stress-induced amplification of the tobacco Tnt1 element, one of the rare active plant retrotransposons. Tnt1 mobility was monitored using the retrotransposon-anchored SSAP strategy that allows the screening of multiple insertion sites of high copy number elements. We have screened for Tnt1 insertion polymorphisms in plants regenerated from mesophyll leaf cells, either via ...

Herein we are the first to report that single-walled carbon nanotubes (SWCNTs) exhibit dual-phase regulation to Arabidopsis mesophyll cells exposed to different concentration of SWCNTs. The mesophyll protoplasts were prepared by enzyme digestion, and incubated with 15, 25, 50, 100 μg/ml SWCNTs for 48 h, and then were observed by optical microscopy and transmission electron microscopy, the react...

Sonicated unilamellar lipid vesicles, consisting of egg lecithin, stearylamine, and cholesterol in 7:2:1 molar ratios, promoted the aggregation of tobacco (Nicotiana glutinosa) protoplasts with the aid of mono- or divalent cations.A reaction mixture containing liposomes (0.4 micromoles lipid per milliliter), 50 millimolar CaCl(2), 0.5 molar mannitol, and 5 x 10(5) protoplasts per milliliter res...

The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breeding. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resis...