SOURCE/DESCRIPTION; A 4.3 lib Mspl fragment of cosmid EFD134 isolated by a HBV-3 oligonucleotide (1) was subcloned into AccI site of pUC18. POLYMORPHISM; Mspl identifies >10 allelic VNTR polymorphism with bands between 1.5 and 2.7 kb. Rsal and TaqI also identify the same VNTR polymorphism. FREQUENCY: With Mspl, 80 % heterozygosity were observed in 85 unrelated Caucasians. NOT POLYMORPHIC FOR: n...

S-layer protein variation from a hexagonally ordered (SbsA; 130 kDa) to a obliquely ordered (SbsB; 98 kDa) protein in Bacillus stearothermophilus PV72 is mediated by an increased oxygen supply. To elucidate the molecular basis of S-layer protein variation in B. stearothermophilus PV72, the sbsB gene, coding for the 98-kDa protein, was cloned by means of inverse PCR technology and sequenced. The...

A gene library of poly(vinyl alcohol) (PVA)-degrading Pseudomonas sp. strain VM15C was constructed in Escherichia coli with the vector pUC18. Screening of this library with a chromogenic PVA dehydrogenase assay resulted in the isolation of a clone that carries the gene (pdh) for the PVA dehydrogenase, and the entire nucleotide sequence of its structural gene was determined. The gene encodes a p...

We have previously published the sequence of the 5' end of the yeast MY01 gene (Watts et aL, 1987). This gene was isolated from a X47.1 genomic library screened with a probe containing the active thiol region of the nematode myosin UNC54 (Karn et ai., 1983). Here we present its complete nucleotide sequence achieved by the dideoxy chain termination method (Sanger, 1977). Templates cloned in pUC1...

Full-length biologically active cDNAs of brome mosaic virus genomic RNAs 1, 2 and 3 were constructed by joining cDNA fragments. The cDNAs were constructed so that, at the 5' ends, unique SnaBI sites were present at the site of initiation of transcription. The cDNAs were inserted between a modified cauliflower mosaic virus (CaMV) 35S RNA promoter and terminator regions derived from CaMV DNA, and...

SOURCE/DESCRIPTION: A 4.1 kl) TaqI fragment from cosmid EFD145 was subcloned into the AccI site of pUC18. POLYMORPHISM: Rsal identifies a two allele polymorphism (Rl : 2.4 kb, M2 : 1.4 and 1.0 kb) with a constant band at 0.9 kb. TaqI also identifies a two allele polymorphism (Tl : 5.0 kb, T2 : 4.1 kb) without a constant band, but this observation has not been verified by pedigree studies. FREQU...

A partial genomic library was prepared in E. coli JM109 using pBR322 as vector and 2.4 kb Sau 3A I chromosomal fragment, encoding a nitroaryl reductase (nbr A) gene, from Streptomyces aminophilus strain MCMB 411. From the library, 2.4 kb fragment was recloned in E. coli JM109 and S. lividans TK64 using pUC18 and pIJ702 as vectors respectively. The recombinant plasmids pSD103 and pSD105 expresse...

The gene for proline iminopeptidase from Lactobacillus delbrueckii subsp. lactis DSM 7290 coding for an enzyme that hydrolyses the synthetic substrate L-prolyl-beta-naphthylamide (Pro-beta NA) was cloned in Escherichia coli. An enzymic plate assay was used to screen for positive clones. The gene, designated pepI, was subcloned into vector pUC18 and sequenced. The nucleotide sequence revealed an...

Knowing the nucleotide sequence of the cholera toxin operon, we designed oligonucleotide primers for its-PCR amplification from local clinical isolates of V. cholerae. The resulting amplification product was cloned in a common pUC18 vector. Subsequently, a part of this operon encoding the cholera toxin Bsubunit (CTB) was reamplified and cloned between the BamH1 and EcoR1 sites of the same ...

DNA fragments from promoter regions of the geminivirus, African cassava mosaic virus, were cloned into pG1, a vector based on pUC18, producing transcriptional fusions with the beta-glucuronidase (GUS) gene and nopaline synthase termination sequence. The activity of each promoter construct was assessed by analysing the transient expression of GUS in Nicotiana clevelandii protoplasts. The results...