Geobacillus thermopakistaniensis strain MAS1 was isolated from a hot spring located in the Northern Areas of Pakistan. The draft genome sequence was 3.5 Mb and identified a number of genes of potential industrial importance, including genes encoding glycoside hydrolases, pullulanase, amylopullulanase, glycosidase, and alcohol dehydrogenases.

The gene encoding a thermoactive pullulanase from the hyperthermophilic anaerobic archaeon Desulfurococcus mucosus (apuA) was cloned in Escherichia coli and sequenced. apuA from D. mucosus showed 45.4% pairwise amino acid identity with the pullulanase from Thermococcus aggregans and contained the four regions conserved among all amylolytic enzymes. apuA encodes a protein of 686 amino acids with...

Streptococcal pullulanases have been recently proposed as key components of the metabolic machinery involved in bacterial adaptation to host niches. By sequence analysis of the Group B Streptococcus (GBS) genome we found a novel putative surface exposed protein with pullulanase activity. We named such a protein SAP. The sap gene is highly conserved among GBS strains and homologous genes, such a...

Abstract Pullulanase is a well-known debranching enzyme that can specifically hydrolyze α-1,6-glycosidic linkages in starch and oligosaccharides, however, it suffers from low stability catalytic efficiency under industrial conditions. In the present study, four residues (A365, V401, H499, T504) lining pocket of Anoxybacillus sp. AR-29 pullulanase (PulAR) were selected for site-directed mutagene...

Cereal Chem. 65(5):424-427 The ratio of A chains to B chains of rice amylopectins from two pairs of action of pullulanase on the /3-limit dextrin and from A and B chains by the near isogenic lines differing in the waxy gene was determined by the action combined action of /3-amylase and pullulanase. Waxy and nonwaxy (lowof pullulanase and of /3-amylase plus pullulanase on the fl-limit dextrins a...