BACKGROUND & OBJECTIVES Purified protein derivative (PPD) is currently the only available skin test reagent used worldwide for the diagnosis of tuberculosis (TB). The aim of this study was to develop a Mycobacterium tuberculosis specific skin test reagent, without false positive results due to Bacillus Calmette-Guerin (BCG) vaccination using recombinant antigens. METHODS Proteins in PPD IC-65...

To determine the mechanism of the purified protein derivative (PPD)-specific hyporesponsiveness in Mycobacterium bovis BCG-vaccinated human T-cell leukemia virus type 1 (HTLV-1)-infected individuals, we examined cytokine production in response to PPD in the following four groups of individuals: (i) HTLV-negative, PPD nonresponders (n = 11; NN); (ii) HTLV-negative, PPD responders (n = 18; NP); (...

We report that M. tuberculosis organisms, but neither PHA nor allogeneic stimulator cells, preferentially activate gamma/delta+ cells within E rosette-purified peripheral blood T cells. gamma/delta+ T cells from purified protein derivative (PPD)-nonimmune healthy donors were enriched by depletion of CD4+ and CD8+ cells; double-negative (DN) cells contained 65-92% gamma/delta+ T cells. Limiting ...

541 animals from three dairy farms () were firstly screened for bovine tuberculosis by tuberculin skin testing, out of which 71 (13.12%) animals were found tuberculin reactors. The serum samples of 71 tuberculin positive104 tuberculin negative and 363 non tuberculin tested animals were then evaluated by Purified Protein Derivative (PPD)-ELISA. PPD-ELISA yielded 57.74%, 8.65% and 24.24% seroposi...

Coinfection with Mycobacterium tuberculosis and human immunodeficiency virus (HIV) is a serious problem, particularly in developing countries. Recently, M. tuberculosis and purified protein derivative (PPD) were demonstrated to induce HIV replication in CD8 T cell-depleted peripheral blood mononuclear cells from HIV-positive, PPD-positive persons but not in cells from PPD-negative persons. The ...

Bacille Calmette-Guérin (BCG) vaccination induces a marked increase in the interferon (IFN)-gamma response to Mycobacterium tuberculosis purified protein derivative (Mtb PPD) in UK adolescents, but not in Malawian adolescents. We hypothesized that Mtb PPD-induced IFN-gamma after BCG vaccination would be similar in infants from these 2 countries. Infants were vaccinated with BCG during the first...

Anti-sheep erythrocyte antiserum suppressed the in vitro immune response of swine spleen cells to sheep erythrocytes. Inhibition of the in vitro anti-sheep erythrocyte response was also observed in neonatal spleen cells, which were obtained from piglets recently fed colostrum. Addition of purified protein derivative (PPD) to spleen cell cultures prepared from animals previously sensitized to My...

Interferon (IFN)-gamma responsiveness to 12 purified protein derivative (PPD) and new tuberculin antigens from 9 species of mycobacteria was assessed, using a whole blood assay, in 616 young adults living in northern Malawi, where Mycobacterium bovis bacille Calmette-Guérin (BCG) vaccination provides no protection against pulmonary tuberculosis. The prevalence of IFN-gamma responsiveness was hi...

OBJECTIVE To identify prognostic factors predicting a fatal outcome in HIV-negative children with neurotuberculosis based on clinical, epidemiological, and laboratory findings. METHOD The clinical records of all in-patients diagnosed with neurotuberculosis from 1982 to 2005 were evaluated retrospectively. The following prognostic parameters were examined: gender, age, close contact with a tub...

A microcytotoxic assay, based on the release of 51Cr from chicken erythrocytes by sensitized chicken lymphoid cells in the presence of the specific antigen, purified protein derivative (PPD), is described. The percentage of antigen-specific lysis was dependent on the lymphoid cell concentration, antigen concentration, time of incubation, and incubation temperature. Maximum antigen-specific lysi...