Sequence analysis of the chromosomal Tn5lacZ flanking regions of the Pseudomonas fluorescens WCS365 competitive root colonization mutant PCL1206 showed that the Tn5lacZ is inserted between genes homologous to bioA and potF. The latter gene is the first gene of the potF1F2GHI operon, which codes for a putrescine transport system in Escherichia coli. The position of the Tn5lacZ suggests an effect...

The mechanism of mammalian polyamine transport is poorly understood. We have investigated the role of plasma-membrane potential (DeltaPsipm) in putrescine and spermidine uptake in ZR-75-1 human breast cancer cells. The rate of [3H]putrescine and [3H]spermidine uptake was inversely correlated to extracellular [K+] ([K+]o) and to DeltaPsipm, as determined by the accumulation of [3H]tetraphenylpho...

A current trapping system for Anastrepha fruit flies uses a two-component food-based synthetic attractant consisting of ammonium acetate and putrescine (1,4-diaminobutane). Development of more effective monitoring programs may be realized through identification of additional attractant chemicals. This study examined response of the Caribbean fruit fly, Anastrepha suspensa (Loew), to putrescine ...

The urinary excretion of the polyamines--putrescine, spermidine, and spermine--was measured in 7 children with Beckwith's syndrome. Putrescine excretion was raised and spermidine excretion reduced. The raised putrescine and the low spermidine ratios were highly significant. These results are consistent with a disturbance in a metabolic pathway under growth hormone-like regulation.

The isolation from the rat ventral prostate of a soluble enzyme preparation producing spermidine, COZ, and methylthioadenosine from putrescine and S-adenosyl-L-methionine is described. This preparation catalyzes limited decarboxylation of S-adenosyhnethionine in the absence of any additional reactants but maximal rates of CO2 release from S-adenosyhnethionine require the presence of putrescine....

The administration to rats of putrescine (750 mumol/kg body wt.) caused in liver, kidney and heart an increase in putrescine at 1 h and in diamine oxidase (EC 1.4.3.6) activity within 3-6 h. An increase in spermidine was observed at 9 h in liver and at 6 h in kidney, whereas in heart there was no change. The increase in diamine oxidase activity by exogenous putrescine was prevented by the admin...

A cytosolic spermidine N-acetyltransferase has been partially purified from livers of rats treated with carbon tetrachloride or thioacetamide. This enzyme formed N’-acetylspermidine when incubated with spermidine and acetyl-coA. The enzyme was also able to acetylate spermine, norspermidine, norspermine and, at a much slower rate, 1,3-diaminopropane. Putrescine, cadaverine, homospermidine, and ...

BACKGROUND AND AIMS It has been suggested that putrescine acts as a growth factor in the gut, but its exact function in some aspects of cellular metabolism is still in question. The aim of the present work was to identify some functions of putrescine in small bowel metabolism. ANIMALS Rats (about 80 g), in groups of five, were given either phytohaemagglutinin- or lactalbumin-containing diets,...