Background: Researchers have indicated that mammalian spermatozoa are characterized by a high proportion of saturated, n-6 and n-3 series of fatty acids (FA). Major FA in rams’ sperm consists of C16, C18:1 and C22:6 (DHA). Little information exists on the effective use of saturated and unsaturated FA for ram whereas lipids have important role in sperm fertility. Our Background was to determine ...

Ejaculated ram spermatozoa, freed from seminal plasma by a dextran/swim-up procedure and exposed to cold shock, were incubated with ram seminal plasma proteins and analyzed by fluorescence markers and scanning electron microscopy. Seminal plasma proteins bound to the sperm plasma membrane modified the functional characteristics of damaged spermatozoa, reproducing those of live cells. Scanning e...

Studies have demonstrated the importance of mitochondria to sperm functionality, as the main source of ATP for cellular homoeostasis and motility. However, the role of mitochondria on sperm metabolism is still controversial. Studies indicate that, for some species, glycolysis may be the main mechanism for sperm energy production. For ram sperm, such pathway is not clear. Thus, we evaluated ram ...

The aim of this study was to investigate the effects of catalase and pre-freezing equilibration during ram sperm cryopreservation on motility and membrane and acrosomal integrity of frozen-thawed semen, as well as conception rate following laparoscopic timedinsemination. Semen was collected from four mature Dorper rams, pooled and diluted in Tris egg-yolk extender basic solution (CON), or this ...

'Forehead to forehead I meet thee, this third time, Moby Dick!' [Ahab (Melville, 1851)] Herman Melville's fictional portrayal of the sinking of the Pequod was inspired by instances in which large sperm whales sank whaling ships by ramming the ships with their heads. Observations of aggression in species of the four major clades of cetacean and the artiodactyl outgroup suggest that head-butting ...

AbADjievA, D., M. Chervenkov, r. StefAnov, n. MetoDiev, e. kiStAnovA, D. kAChevA and e. rAyChevA, 2014. effect of breeding season on the kinematic parameters and morphology of ram’ sperm from synthetic population bulgarian milk sheep breed. Bulg. J. Agric. Sci., 20: 967-972 the aim of this study was the investigation of the breeding season effect on the kinematic and main spermatological parame...

approved: Redacted for privacy Philip D. Whanger Following initial intramuscular injections of 500 pCi 75 Se, semen was collected periodically by electroejaculation over a 63 day period from a selenium-deficient and a selenium-adequate ram. Ten and three days prior to necropsy, second and third injections of 500 11Ci were given. Testes, accessory glands, and semen were obtained from a bull inje...

Mammalian sperm plasma membranes, in contrast to those of mammalian somatic cells, exhibit a significant fraction of lipid that does not diffuse laterally in the plane of the membrane. This nondiffusing fraction results from lipid-lipid interactions. Similar nondiffusing fractions are found in mixed-lipid model systems that contain coexistent gel and fluid domains. These results suggest that th...