Background and Objective: Escherichia coli bacteria are one of the most important bacterial races causing urinary tract infection, which is responsible for 90% causes infection originated in human intestine, this Gram- Negative facultative aerobic that moves by peripheral flagella belongs to Enterobacteriaceae.
 Materials methods: The were isolated from UTI, after they diagnosed using ligh...

The objective of the present study was to conduct DNA based genotyping analysis for hybrid identification in maize (Zea mays L.) using DNA fingerprinting technology. To achieve this random amplified polymorphic DNA (RAPD) analysis was employed. A total of 40 random tenmer primers were surveyed. Of these, OPR-03, OPR-11 and OPT-06 were polymorphic which not only verified the purity among three l...

The present study was conducted to evaluate the genetic diversity and relationships of 5 Egyptian indigenous strains (Fayoumi, Dokki-4, Golden Montazah, Silver Montazah, and El-Salam) using 10 random amplification of polymorphic DNA (RAPD) primers. The gel analyses were performed using GelCompare II where the matrix was constructed followed by the cluster analysis for the matrices. Three hundre...

Turkey has very rich quince genetic resources, and the country currently dominates world quince production. In particular, the northeastern part of the country has notable Cydonia oblonga Mill. germplasm. Authenticating the identity of germplasm resources of C. oblonga Mill. would be of great value for breeding practices. In the present study, genetic variations of 14 C. oblonga Mill. genotypes...

Various organisms have been characterized by molecular methods, including fungi of the genus Cryptococcus. The purposes of this study were: to determine the discriminatory potential of the RAPD (Random Amplified Polymorphic DNA) primers, the pattern of similarity of the Cryptococcus species, and discuss their useful application in epidemiological studies. We analyzed 10 isolates of each specie/...

OBJECTIVE To study primer sequences (1060, 1247, 1254, 1281, 1283, and 1290) for random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). METHODS Twenty-four clinical Serratia marcescens (S. marcescens) isolates were obtained over a 6-month period from April 2002 to September 2002 from hospitals in the Fars province of Iran. Six primers were used due to S. marcescens genome prop...

Reproducible and target-specific polymerase chain reaction (PCR) amplification relies on several interrelated factors of which primer design is central. Here, we describe new free bioinformatics software, the FastPCR which was developed, and continues to be updated, based on detailed experimental studies of PCR efficiency for the optimal design of primers and probe sequences and for repeat sear...

in the present study, random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) markers were used to estimate genetic diversity and relationships among 35 cluster bean [cyamopsis tetragonoloba (l.) taub] genotypes. for rapd analysis, 20 random primers were used which amplified 164 bands, 147 of which were polymorphic with an average polymorphism of 90.3%. the amplified pro...