introduction: streptomyces species are mycelial, aerobic gram-positive bacteria that are isolated from soil and produce a diverse range of antibiotics. streptomyces griseus produces the antibiotic, streptomycin and forms spores even in a liquid culture. the gene cluster for the production of streptomycin antibiotic contains strr gene that encodes strr, a pathway-specific regulator. then, this p...

background and objectives: the significant rise in food borne infections is mainly caused by campylobacter spp., salmonella serovars and verotoxigenic escherichia coli . as the emerging food borne pathogens cause disease, more studies have been conducted for rapid detection of these pathogens. the combination of immunomagnetic separation and polymerase chain reaction (ims-pcr) is the most accur...

A rapid coliphage detection assay was developed, based on the phage-induced release of beta-galactosidase from cells of Escherichia coli. The assay could detect as few as five coliphage per sample without an overnight incubation period. The range of acceptable assay parameters was identified.

A reliable method is presented for rapidly screening human sera to detect macroamylasemia. A mixture of the serum to be tested, Blue Dextran, and cytochrome c is filtered through a small column of cross-linked dextran gel (Sephadex G-100). Ainylase in the effluent fractions is detected by incubation with amylose and subsequent addition of iodine. Macroamylase, if present, is found in the blue-c...

methods genomic dna of cultured s. agalactiae was prepared and loop-mediated isothermal amplification (lamp) primers were designed based on the camp gene in bacteria. the optimum primer set was selected based on the reaction speed and specificity. the reaction result was monitored visually. the sensitivity and specificity of the lamp method were evaluated and compared with polymerase chain reac...

adenoviruses (advs) types 40 and 41 are the causative agents of diarrhea in children. hence, rapid sensitive and specific detection of these viruses are of clinical importance. the customary methods such as propagation of virus in cell culture suffer from limitations. detection of immobilized amplified products in a one phase system (diapops) method has the potential to overcome these problems....