Increasing amounts of evidence suggest noninflammatory roles for growth factor and cytokines in development and differentiation. Leukemia inhibitory factor (LIF) belongs to a gp130 pleiotropic family of growth factors that has recently been shown to enhance the survival of rat testicular gonocytes and Sertoli cells. In this study, we show the expression of gp130 and LIF messenger RNAs (mRNAs) i...

Ca2+-dependent protein phosphorylation and the role of calmodulin in this process was investigated in subcellular fractions of primary cultures of rat Sertoli cells. Significant Ca2+/calmodulin-dependent protein phosphorylation in Sertoli cells was restricted to the cytosol fraction. The calmodulin dependence of these effects was confirmed by using the calmodulin inhibitor trifluoperazine. One ...

It is currently believed that the fertility level of the adult mammalian testis is related to the total number of Sertoli cells, which is established in the early prepubertal life. We have previously reported that, in an in-vitro system, terminal Sertoli cell proliferation is sustained by activin A in concert with FSH. In this paper, we have addressed the question of whether this activin A effe...

A cocultivation chamber and two types of permeable substrates have been used to study: (1) the culture patterns of rat Sertoli and peritubular cells, and Sertoli cells cocultured with spermatogenic cells or peritubular cells; and (2) the polarized secretion of Sertoli cell-specific proteins transferrin, S70 and S45-S35 heterodimeric protein. Substrates included a nylon mesh (with openings of 10...

introduction: morphology and ultra-structure of myoid and sertoli cells were studied in patients with non-obstructive azoospermia who were admitted to testicular sperm extraction (tese) and intracytoplasmic sperm injection (icsi), by transmission electron microscopy (tem). materials and methods: 12 biopsies of testicular tissues from patients with non-obstructive azoospermia referred to royan i...

spermatogonial stem cells (sscs) maintain spermatogenesis throughout life in the male. maintenance of sscs and induction of spermiogenesis in vitro may provide a therapeutic strategy to treat male infertility. this study investigated in vitro differentiation of mouse sscs in presence or absence of sertoli cells, hormones and vitamins. spermatogonial populations were enriched from testes of 4-6 ...

Background Sertoli cells located in seminiferous tubular basement membrane and surrounds different types of germ cell and they were only somatic cell type that directly contacts germ cells. These cells have Several role like support activities, nutrition of germ cells.During spermatogenesis, expression of SOX3 is necessary for differentiation of spermatogonia A. SOX3 is a transcription factor, ...

OBJECTIVES We sought to study the renoprotective effect of cotransplanted allogeneic testicular Sertoli cells on renal acute rejection in rats. MATERIALS AND METHODS A renal acute rejection model using kidneys from Sprague-Dawley (n=30) transplanted into Wistar rats (n=30) was constructed. The rats were randomly divided into 3 groups: (1) the cyclosporine group, which was treated with daily h...