background: targeting of cd20 antigen with monoclonal antibodies has become the mainstay in the treatment of non-hodgkin's lymphomas and immunotherapeutic depletion of malignant b cells. accessibility of antigen is one of the crucial factors in development of monoclonal antibodies against this antigen. one major problem in expression of full length cd20 is aggregation and misfolding. there...

background: although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. objective: the aim of this study was to clone the first 1100 bp of the c. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...

Rabies virus nucleoprotein (N protein) encapsidates genomic RNA of the virus and forms the viral ribonucleoprotein complex. These N proteins represent highly organized structures which activate proliferation of B cells and production antibodies against the N protein. In addition to the B cell, the rabies virus N protein has been shown to induce potent T helper cell responses resulting in a long...

Objective(s): Helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. The vacuolating cytotoxin (VacA) is an effective virulence factor involved in gastric injury. The aim of this study was to construct a recombinant protein containing antigenic region of VacA gene and determine its antigenicity.   Materials and Methods: The antigenic region of V...

background: the mediterranean type of kala-azar is occurred in different parts of iran and caused by leishmania infan tum. a rapid and valid test for early detection of visceral leishmaniasis in human would be highly desirable because it could de crease mortality rate of the disease. in this study, we aimed to compare the results of k39sub antigen with an commercial immu nochromatographic dipst...

Updated and revised versions of COVID-19 vaccines are vital due to genetic variations the SARS-CoV-2 spike antigen. Furthermore, that safe, cost-effective, logistic-friendly critically needed for global equity, especially middle- low-income countries. Recombinant protein-based subunit against have been reported using receptor-binding domain (RBD) prefusion trimers (S-2P). Recently, a new versio...

antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. a promising alternative to conventional antibodies are the heavy chain antibodies (vhh) of camelidae having short length, high solubility and stability are preferred to other antibody derivatives. in this study, our goal was product...

Background and Aims: Influenza is an acute respiratory illness that is caused by a virus belonging to Orthomyxoviridae family. This virus spreads rapidly every year in cold season and leads to morbidities and mortalities especially in adults and children, which causes billions of dollars of economic losses. Accordingly, development of a rapid, sensitive and inexpensive laboratory diagnosis base...

background and objectives: the obligate intracellular bacterium chlamydia trachomatis causes sexually transmissible diseases in human. timely and sensitive detection of this pathogen is very important. there are many cross-reactions in bacteriological and serological methods in detection of this type of pathogens. the aim of this study was to achieve a more specific antigen for serological test...

The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...