The ascorbate (AsA)–glutathione (GSH) metabolism pathway is an important antioxidant system in cadmium (Cd) detoxification; the AsA–GSHpathway generally regulated by a specific set of functional genes. However, transcription factors involved AsA–GSH have yet to be identified. Herein, we transformed heat shock factor SpHsfA4c from Sedum plumbizincicola into Populus. × canescens. Under 100 μM CdC...

Glutathione, γ-glutamylcysteinylglycine, exists abundantly in nearly all organisms. Glutathione participates in various physiological processes involved in redox reactions by serving as an electron donor/acceptor. We found that the abundance of total glutathione increased up to 60% in resistant wheat plants within 72 hours following attack by the gall midge Mayetiola destructor, the Hessian fly...

Glutathione (GSH) and GSH-dependent enzymes play a key role in cellular detoxification processes that enable organism to cope with various internal and environmental stressors. However, it is often not clear, which components of the complex GSH-metabolism are required for tolerance towards a certain stressor. To address this question, a small scale RNAi-screen was carried out in Caenorhabditis ...

Reduced glutathione (GSH) and activities of several glutathione-related enzymes were measured in two 9L rat brain tumor cell lines with differing sensitivities to both 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and nitrogen mustard. GSH, measured by a specific high-performance liquid chromatographic method, was found to be approximately twice as high in 9L cells sensitive to BCNU but resistant...

Glutathione export from uninfected human erythrocytes was compared with that from cells infected with the malaria parasite Plasmodium falciparum using two separate methods that distinguish between oxidized (GSSG) and reduced (GSH) glutathione. One involved enzymatic recycling with or without thiol-masking; the other involved rapid derivatization followed by HPLC. Glutathione efflux from uninfec...

Aims: The present research was done to evaluate the antioxidant activity of aqueous extract Withania somnifera (Ashwagandha) in equine. Herbal antioxidants have gained popularity due their potency and safety, oxidative stress is a condition that more prevalent horses performing physical activities.
 Methods: Twenty clinically healthy were selected equally divided into treatment control gro...

Background: Several studies have demonstrated that elevated Aluminium (Al) levels contribute to Alzheimer or ALS Multiple sclerosis pathophysiology. Aim: we have described three cases of patients who had extremely elevated Aluminium levels (Al) (before any treatment) by unknown causes. Results: These patients reduces 48 % their Aluminium levels and rise (67 %) GSH (Glutathione: reduced form) le...

Abstract α-Aminoadipic acid (Aaa) is an intermediate product in the Lys catabolism, on saccharopine pathway. Since content was increased by various abiotic stress factors affecting redox environment, a glutathione-dependent regulation of Aaa metabolism assumed which not tested earlier. The effect modified size and state glutathione pool (reduced + oxidised, GSH GSSG) related amino transcript le...

OBJECTIVES We investigated whether glutathione (GSH), a reduced thiol that modulates redox state and forms adducts of nitric oxide (NO), improves endothelium-dependent vasomotion and NO activity in atherosclerosis. BACKGROUND Endothelial dysfunction and reduced NO activity are associated with atherosclerosis and its clinical manifestations such as unstable angina. METHODS In the femoral cir...