1. Catabolite repression of beta-galactosidase and of thiogalactoside transacetylase was studied in several strains of Escherichia coli K 12, in an attempt to show whether a single site within the structural genes of the lac operon co-ordinately controls translational repression for the two enzymes. In all experiments the rate of synthesis of the enzymes was compared in glycerol-minimal medium ...

Aerobic repression of the hypoxic genes of Saccharomyces cerevisiae is mediated by the DNA-binding protein Rox1 and the Tup1/Ssn6 general repression complex. To determine the DNA sequence requirements for repression, we carried out a mutational analysis of the consensus Rox1-binding site and an analysis of the arrangement of the Rox1 sites into operators in the hypoxic ANB1 gene. We found that ...

This article narrates the collaborative composition of a song in response to fatal repression protests Cali, Colombia, 2021, It simultaneously provides an analysis both final recording and mediated interactions people involved its creation. I argue that such online musical collaboration can be fitting situations which direct protest may put participants at risk. The sounds song, incorporating e...

micrornas (mirnas) are small preserved non-coding rna molecules that regulate gene expression post-transcriptionally by targeting the 3' utr of mrnas for translational repression or degradation. the rising evidence has established the significant role of mirnas within the regulation of immune system and the prevention of autoimmunity. mir-146a has obtained an importance as a modulator of d...

Both transcriptional activation and repression have essential functions in maintaining proper spatial and temporal control of gene expression. Although Wnt signalling is often associated with gene activation, we have identified several directly repressed targets of Wnt signalling in Drosophila. Here, we explore how individual Wnt target genes are specified for signal-induced activation or repre...

The mechanism by which the internal lacZ gene sequence O2 influences lac repression was investigated by using in vivo footprinting of operon mutants. Quantitative in vivo binding curves show that O2 strengthens by approximately 3-fold repressor binding to O1 that is located 400 base pairs upstream at the transcription start site. The internal O2 sequence also contributes to repression by a seco...

Expression of the Bacillus subtilis hut operon is subject to regulation by catabolite repression. A set of hut-lacZ transcriptional fusions was constructed and used to identify two cis-acting sites involved in catabolite repression. The hutOCR1 operator site lies immediately downstream of the hut promoter and weakly regulates hut expression in response to catabolite repression. The downstream h...

This article discusses how state suppression of human rights activism and restrictions on civil society are likely to impact the implementation United Nations’ International Decade Indigenous Languages (2022–2032, hereafter “the Decade”). We focus China, India, Indonesia, three most populous linguistically diverse countries in Asia. Drawing a range reports from organizations materials academic ...