resistance gene analog polymorphism (rgap) markers linked to fusarium head blight resistance (fhb) and co-localize with qfhs.ndsu-3bs were identified using f3 plants and f3:5 lines derived from a ‘wangshuibai’ (resistant) / ‘seri82’ (susceptible) cross. the mapping populations were genotyped using 50 degenerate primers designed based on the known r genes. out of the 50 designed primer combinati...

Pek et al. describe how a stable, intron-derived RNA regulates the expression of its host gene during Drosophila embryogenesis. Once they are spliced out of nascent mRNAs, noncoding intron sequences are usually degraded rapidly. At least in Xenopus eggs and human cell lines, however, some intronic RNAs persist for long periods, although the function of these stable intronic sequence (sis) RNAs ...

Linkage maps developed from known-function genes can be valuable in the candidate gene mapping approach. A set of 121 expressed sequence tagged site (E-STS) primer pairs were tested on a framework genetic linkage map of apple (Malus x domestica Borkh.) constructed using simple sequence repeats (SSRs) and randomly amplified polymorphic DNA (RAPD) markers. These known-function gene markers, E-STS...

The growth of Arabidopsis thaliana is quantitatively regulated by the phytohormone gibberellin (GA) via two closely related nuclear GA-signaling components, GAI and RGA. Here we test the hypothesis that GAI and RGA function as "GA-derepressible repressors" of plant growth. One prediction of this hypothesis is that plants lacking GAI and RGA do not require GA for normal stem growth. Analysis of ...

In this paper, Protein Tertiary Structure Prediction using Evolutionary Algorithms (EAs) such as SelfAdaptive Differential Evolution (SaDE) and Real-coded Genetic Algorithm (RGA) are discussed. RGA is implemented with various crossover and mutation operators. The algorithms are tested on a peptide Metenkephalin. The energy functions used are ECEPP/2 and ECEPP/3 force fields. SaDE and RGA with d...

Therefore, RGAs and RGLs have the potential to serve as closely linked markers for marker-assisted breeding Conserved motifs within resistance genes have been utilized in strategies, or they could be candidate R-genes thempolymerase chain reaction (PCR)-based strategies to isolate resistance gene analogs (RGAs) and resistance gene-like (RGLs) sequences from selves. many plant species. RGAs have...

The Arabidopsis (Arabidopsis thaliana) SPINDLY (SPY) protein negatively regulates the gibberellin (GA) signaling pathway. SPY is an O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) with a protein-protein interaction domain consisting of 10 tetratricopeptide repeats (TPR). OGTs add a GlcNAc monosaccharide to serine/threonine residues of nuclear and cytosolic proteins. Determination of the...

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was closely linked with the RFLP probe Xpsr...