This infographic outlines evidence-based recommendations on COVID-19 reverse transcriptase PCR (RT-PCR) testing in elite sport settings, aiming to protect personal and population health, acknowledging resources expertise that are often available sport. Public health vary by country region, protocol decisions should be made consultation with relevant public authorities. An expert, multidiscip...

MR303 is a newly released Malaysian rice cultivar that comes with special traits as it can be cultivated in less fertile soils. Previous studies stated miR164b key molecule responsible for regulating drought stress tolerance many varieties. Thus, this study aims to identify the presence and regulatory role of variety using both computational experimental approaches. The stem-loop structure (pre...

Recently, a novel technique for "real time" quantitative Reverse Transcriptase-PCR which measures PCR-product accumulation during the exponential phase of the PCR reaction using a dual-labelled fluorogenic probe, has been developed. This method allows direct detection of PCR-product formation by measuring the increase in fluorescent emission continuously during the PCR reaction. Here we present...

Syphilis is diagnosed by serologic testing or by identification of the causative agent, Treponema pallidum. The bacterium has historically been detected in clinical specimens by dark-field microscopy, immunostaining with polyclonal or monoclonal antibodies, or the rabbit inoculation test (RIT). RIT is considered to be very sensitive and specific, although it is available only in research settin...

The genetic capacity to fix gaseous nitrogen (N) is distributed among diverse diazotrophs belonging to the Bacteria and Archaea. However, only a subset of the putative diazotrophs present actively fix N at any given time in the environment. We experimentally tested whether the availability of carbon and inhibition by oxygen constrain N fixation by diazotrophs in coastal seawater. The goal was t...

High-throughput real-time quantitative reverse transcriptase polymerase chain reaction (qPCR) is a widely used technique in experiments where expression patterns of genes are to be profiled. qPCR is widely accepted as the ”gold standard” for analysis of gene expression. Recent technological advances have greatly expanded the total number of genes that can be analyzed in a single assay; qPCR exp...