Mycobacterium tuberculosis and M. bovis infect animals and humans. Their epidemiology in developed and developing countries differs, owing to differences in the implementation of preventive measures (World Health Organization, 1999). Identification and differentiation of these closely related mycobacterial species would help to determine the source, reservoirs of infection, and disease burden d...

background: we aimed to develop a pcr-rflp assay based on available sequences of putative vertebrate hosts to iden­tify blood meals ingested by field female sand fly in the northwest of iran. in addition, the utility of pcr-rflp was compared with elisa as a standard method. methods: this experimental study was performed in the insect molecular biology laboratory of school of public health, tehr...

OBJECTIVES Rapid and accurate detection of Brucella abortus and Brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. This study reveals a new method in detection of clinical samples of brucellosis using real-time PCR and high-resolution melt (HRM) curve analysis. MATERIALS AND METHODS 160 brucellosis suspicious samples with more than 1...

Traditional methods for confirming the identity of herpes simplex virus (HSV) isolates use restriction fragment length polymorphism (RFLP). However, RFLP is less amenable to high-throughput analyses of many samples, and the extent to which small differences in RFLP patterns distinguish between different viral strains remains unclear. Viral HSV type 2 (HSV-2) DNA isolates from 14 persons experie...

BACKGROUND Dermatophytes are the most common causative agents of superficial mycoses. Species identification of these fungi is important from therapeutic and epidemiological point of wive. Traditional approaches for identification of dermatophytes at the species level, relying on macroscopic and microscopic features of the colonies, usually are time-consuming and unreliable in many circumstance...

Implication for health policy/practice/research/medical education: Molecular virology methods are developing rapidly and allow new insights into molecular epidemiology of viral infections. Despite the recent introduction of advanced methodology to genotype hepatitis B virus, using old but rapid and inexpensive genotyping tools like restriction fragment length poly-morphism have been utilized wi...

Restriction fragment length polymorphisms (RFLPs) are being used to construct complete linkage maps for many eukaryotic genomes. These RFLP maps can be used to predict the inheritance of important phenotypic loci and will assist in the molecular cloning of linked gene(s) which affect phenotypes of scientific, medical and agronomic importance. However, genetic linkage implies very little about t...

Background: Microsomal epoxide hydrolase 1 (EPHX1) plays an important role in both activation and detoxification of polycyclic aromatic hydrocarbons (PAH) amines. Polymorphism EPHX1 His139Arg susceptibility to lung cancer has been reported with inconsistent outcomes. Aim this study was analyze the relationship between polymorphism smokers.Method: Consecutive sampling case-control applied. Genot...

Chlamydia trachomatis is one of the most common sexually transmitted infections worldwide. Based on sequence variation in the ompA gene encoding the major outer membrane protein, the genotyping scheme distinguishes 17 recognized genotypes, i.e. A, B, Ba, C, D, Da, E, F, G, H, I, Ia, J, K, L1, L2, and L3. Genotyping is an important tool for epidemiological tracking of C. trachomatis infections, ...