The process of jatropha oil transesterification was studied for biodiesel production. Rhizomucor miehei lipase (RML) immobilized on 3-aminopropyltriethoxysilane functionalized magnetic Fe3O4 employed as a biocatalyst. confirmed by scanning electron microcopy (SEM), Fourier transform infrared spectra (FT-IR), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and confocal las...

Technical-grade oleic acid esters of sucrose and fructose were prepared using solvent-free biocatalysis at 65 ̋C, without any downstream purification applied, and their physicochemical and bioactivity-related properties were evaluated and compared to a commercially available sucrose laurate emulsifier. To increase the conversion of sucrose and fructose oleate, prepared previously using solvent-...

BACKGROUND Use of enzymes in low water media is now widely used for synthesis and kinetic resolution of organic compounds. The frequently used enzyme form is the freeze-dried powders. It has been shown earlier that removal of water molecules from enzyme by rinsing with n-propanol gives preparation (PREP) which show higher activity in low water media. The present work evaluates PREP of the lipas...

Enantioselectivity of enzyme catalysis is often rationalized via active site models. These models are constructed on the basis of comparing the enantiomeric excess of product observed in a series of reactions which are conducted with a range of homologous substrates, typically carrying various side chain substitutions. Surprisingly the practical application of these simple but informative 'pock...

Lipoaminoacids (LAA) are an important group of biosurfactants, formed by a polar hydrophilic part (amino acid) and hydrophobic tail (lipid). The gemini LAA structures allow the formation supramolecular complex with bioactive molecules, like DNA, which provides them good transfection efficiency. Since lipases naturally involved in lipid protein metabolism, they alternative to chemical production...