Sections of Rhodospirillum rubrum cells from cultures of different ages have been examined to obtain information on the development of chromatophores in this organism. Cells from the 12-hour cultures studied contain neither distinct invaginations of the cytoplasmic membrane nor distinct chromatophores. The first structures that can be related to chromatophore development occur peripherally in t...

Reconstituted LH1 complexes were prepared using the LH1 subunit-type complexes, isolated from the purple photosynthetic bacterium Rhodospirillum (Rs.) rubrum, and purified all-trans spirilloxanthin. Stark absorption spectra of spirilloxanthin bound to both the native and reconstituted LH1 complexes were compared in different polarization angles (χ) against the external electric field. From the ...

We develop a random matrix model approach to study static disorder in pigment-protein complexes in photosynthetic organisms. As a case study, we examine the ring of B850 bacteriochlorophylls in the peripheral light-harvesting complex of Rhodospirillum molischianum, formulated in terms of an effective Hamiltonian describing the collective electronic excitations of the system. We numerically exam...

6 Kochakian, C. D., and D. G. Harrison, Endocrinology, 70, 99 (1962). 7 Mueller, G. C., A. M. Herranen, and K. F. Jervell, Recent Prog. Hormone Research, 14, 95 (1958). 8 Mueller, G. C., J. Gorski, and Y. Aizawa, these PROCEEDINGS, 47, 164 (1961). 9 Wilson, J. D., J. Clin. Invest., 41, 153 (1962). 10Kalman, S. M., and P. M. Opsahl, Biochim. Biophys. Acta, 49, 614 (1961). 11 Wilson, J. D., Bioch...

1. A pathway for the synthesis of hexose from succinate by Rhodospirillum rubrum is proposed. 2. With 2,3-(14)C(2)-labelled succinate and fumarate as substrates in experiments with chromatophores and a soluble enzyme fraction of R. rubrum it was found that the products of succinate metabolism by the extracts were the same as in whole cells. It was also found that the light-dependent oxidation o...

The purple photosynthetic bacterium Rhodospirillum centenum is capable of forming swarm colonies that rapidly migrate toward or away from light, depending on the wavelength of excitation. To identify components specific for photoperception, we conducted mini-Tn5-mediated mutagenesis and screened approximately 23,000 transposition events for mutants that failed to respond to either continuous il...

Detailed studies of the properties of carotenoids isolated from diphenylamine-inhibited cultures of Rhodospirillum rubrum have revealed a number of novel structures that indicate new features of carotenoid biosynthesis in the photosynthetic bacteria. Both neurosporene and 7,8,11,12-tetrahydrolycopene undergo hydration, methylation and dehydrogenation to yield spheroidene and 11',12'-dihydrosphe...

The amino acid sequence of cytochrome c' from the purple photosynthetic bacterium Rhodospirillum rubrum S1 has been determined and is consistent with homology to cytochrome c' from the nonphotosynthetic bacterium Alcaligenes sp. NCIB 11015. There is 29% identity in the chosen alignment of these two proteins. R. rubrum cytochrome c' is composed of a single peptide chain of 126 amino acid residu...

The amino acid sequence of cytochrome c’ from the purple photosynthetic bacterium Rhodospirillum rubrum Sl has been determined and is consistent with homology to cytochrome c’ from the nonphotosynthetic bacterium Alcaligenes sp. NCIB 11015. There is 29% identity in the chosen alignment of these two proteins. R. rubrum cytochrome c’ is composed of a single peptide chain of 126 amino acid residue...