Evaluation of gene expression in urinary sediment has been considered as a promising non-invasive approach for biomarker identification of kidney diseases. Nonetheless, there are several challenges in extraction of RNA from this valuable source of biomarkers, mostly because of the factors that have influence on quality of isolated RNA such as low cellular content. Accordingly, we compared the q...

background : gb virus c (gbv-c) is a flavivirus that was characterized in 1995. the prevalence of gbv-c rna in hiv-in­fected subjects has not been previously studied in iran and was therefore determined. methods: we have investigated serum samples of 80 patients from imam khomeini hospital, tehran, iran, whose hiv in­fection was confirmed in our laboratory by western-blotting. we used nested-pc...

noncoding ribonucleic acids micro-rna is involved in the regulation of gene expression have major roles in the post-transcriptional level. a micro-rna alone several causes down regulation of mrna transcript of the target. thus, small changes in the expression of a micro rna may lead to significant changes in gene expression are different. micro- rna as key regulators of immune cell lineage diff...

leishmania is a protozoan parasite belonging to the family trypanosomatidae, which is found among 88 different countries. the parasite lives as an amastigote in vertebrate macro­phages and as a promastigote in the digestive tract of sand fly. it can be cultured in the laboratory us­ing appropriate culture media. although the sexual cycle of leishmania has not been observed during the promastigo...

RNA processing is essential factor for synthesis of functional and structural proteins in eukaryote cells. In eukaryote organisms it will be initiated with transcription of DNA in nucleolus and terminated to mRNA translation in cytoplasm, finally mRNA degraded. Protein synthesis followed as different steps, includes 5' capping, poly adenylating, processing and transferring from nucleolus to cyt...

Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...

RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extracti...