نتایج جستجو برای: rna sequencing
تعداد نتایج: 355158 فیلتر نتایج به سال:
Nanopore direct RNA sequencing assisted by pseudouridine- and m 5 C-specific bisulfite treatment is a technology that allows for epitranscriptomic modifications with the possibility of quantitative assessment.
RNA sequencing (RNA-Seq) is a powerful tool for analyzing the identity of cellular RNAs but is often limited by the amount of material available for analysis. In spite of extensive efforts employing existing protocols, we observed that it was not possible to obtain useful sequencing libraries from nuclear RNA derived from cultured human cells after crosslinking and immunoprecipitation (CLIP). H...
RNA Sequencing (RNA-Seq) is the sequencing and analysis of transcriptomes. The main purpose RNA-Seq to find out presence quantity in an experimental sample under a specific condition. Essentially, raw sequence data was massive. It can be as big hundreds Gigabytes (GB). This massive always makes processing time become longer take several days. A multicore processor speed up program by separating...
Introduction: The emergence of single-cell RNA-sequencing (scRNA-seq) technology has provided new information about the structure of cells, and provided data with very high resolution of the expression of different genes for each cell at a single time. One of the main uses of scRNA-seq is data clustering based on expressed genes, which sometimes leads to the detection of rare cell populations. ...
The medicinal plant, Trachyspermum ammi is a rich source of active pharmaceutical ingredients with pharmaceutics effects. Microsatellite markers play a key role in the genome and gene expression, especially in secondary metabolite biosynthesis in medicinal plants. For the first time, transcriptome sequencing of this herb medicine was carried out to identify the microsatellite markers of this sp...
High-throughput sequencing has been used to study post-transcriptional regulations, where the identification of protein-RNA binding is a major and fast-developing sub-area, which is in turn benefited by the sequencing methods for whole-transcriptome probing of RNA secondary structures. In the study of RNA secondary structures using high-throughput sequencing, bases are modified or cleaved accor...
It has recently been established that synthesis of double-stranded cDNA can be done from a single cell for use in DNA sequencing. Global gene expression can be quantified from the number of reads mapping to each gene, and mutations and mRNA splicing variants determined from the sequence reads. Here we demonstrate that this method of transcriptomic analysis can be done using the extremely low le...
Human skin harbours a large proportion of the resident memory T (Trm) cells body. Trm promote immune homeostasis and exert anti-microbial anti-cancer function in tissue. We recently demonstrated that are permanently depleted people living with HIV (PLWH) low nadir possibly replaced by blood-derived cells. linked loss PLWH increased risk developing cutaneous mucosal cancer. The T-cell receptor (...
Large volumes of high-throughput sequencing data have been submitted to the Sequencing Read Archive (SRA). The lack experimental metadata associated with makes reuse and understanding quality very difficult. In case RNA (RNA-Seq), which reveals presence quantity in a biological sample at any moment, it is necessary consider that gene expression responds over short time interval (several seconds...
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