The effects of major mineral salts, L-glutamine, myo-inositol and carbon source on shoot bud proliferation of date palm (Phoenix dactylifera L.) cv. Mejhoul were evaluated. Different concentrations of ammonium nitrate (NH4NO3; 550, 825 or 1650 mg/L), potassium nitrate (KNO3; 633.3, 950 or 1900 mg/L), calcium chloride dehydrate (CaCl2·2H2O; 147, 220 or 440 mg/L), potassium dihydrogen phosphate (...

Factors like slow and seasonal multiplication and infection with pathogens have constrained the use of conventional plant propagation methods, thus lead to development of new and novel methods of propagation like in vitro multiplication which ensures the production of virus and disease free elite planting material in large numbers. In the present work, in vitro regeneration protocol has been st...

Establishment of an efficient explants surface disinfection protocol is essential for in vitro cell and tissue culture as well as germplasm conservation, such as the case of Grapevine (Vitis spp.) culture. In this research, different procedures for disinfection and regeneration of field-grown grapevine cv. 'Flame seedless' axillary buds were evaluated. The buds were disinfected using either NaO...

Gene sequences are routinely used to determine the topologies of unrooted phylogenetic trees, but many of the most important questions in evolution require knowing both the topologies and the roots of trees. However, general algorithms for calculating rooted trees from gene and genomic sequences in the absence of gene paralogs are few. Using the principles of evolutionary parsimony (EP) (Lake J...

Peanut (Arachis hypogaea L.) and cotton (Gossypium hirsutum) plants were grown for 4 weeks in saline, isoosmotic rooting substrates with different proportions of K and Na. Isoosmotic media did not affect growth (except at the highest external K concentrations) or estimates of intracellular osmotic pressure in expanding leaves (i.e. osmotic pressure of leaf sap and intracellular osmotic pressure...

After about 20 days, hypocotyl cuttings from 20-day-old loblolly pine (Pinus taeda L.) seedlings rooted easily in the presence of the auxin indole-3-butyric acid (IBA), with roots forming directly from xylem parenchyma. In contrast, woody cuttings from 1-2-year-old hedged seedlings formed roots indirectly from callus tissue in 60-90 days, but IBA had little effect on rooting. Variation in rooti...

Semi-hardwood cuttings of ‘Frantoio’(high rooting ability) and ‘Gentile di Larino’(low rooting ability) cultivars were obtained from one-year-old olive shoots sampled in mid-August. Semi-hardwood cuttings w e re dipped in H2O2 (0% control and 3.5% w/v) and IBA (2000 and 4000 ppm) solutions before rooting in greenhouse equipped with an automatic mist system. After 35 days, 50% of semi-hardwood c...

Adventitious root formation in plant cuttings is influenced by many endogenous and environmental factors. Leaf photosynthesis during rooting of leafy cuttings in hard to root species can contribute to supply carbohydrates to the intensive metabolic processes related to adventious root formation. Light intensity during rooting is artificially kept low to decrease potential cutting desiccation, b...

Ildole 3 butyric acid (IBA) at 8rng l i induced, vigorous rooting in Bupleurum falcatum L. (Saiko) root culture in B5 medium after about 2weeks, when it was added at the time when detached roots were transferred to fresh medium. The rooting activity markedly decreased with delay of IBA addition after root 2transplantation, and was hardly observed when IBA was added at the 7th day. Stresses such...

In forest production systems, vegetative propagation of elite clones through adventitious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts have been made to identify markers or/and regulato...