We describe a statistical method for predicting most likely reactions in a biochemical reaction network from the longitudinal data on species concentrations. Such data is relatively easily available in biochemical laboratories, for instance, via the popular RTPCR technology. Under the assumed kinetics of the law of mass action, we also propose the data-based procedures for (i) estimating the pr...

A reverse transcription-polymerase chain reaction (RTPCR) test was developed to differentiate between H1 and H3 subtypes of swine influenza virus (SIV). The sensitivity and specificity of this test was evaluated by comparing the results of the PCR test with subtyping results of an immunological assay performed at the National Veterinary Services Laboratories (NVSL). The test was performed on 68...

<p>Tuberculosis can affect any organ. Orbital tuberculosis is very uncommon. The involvement of adnexa orbit especially the eyelid rare even in endemic region. Many patients with orbital Tuberculosis doesn’t have concomitant other system by tuberculosis. We report a 13-year-old girl presented complaints painless progressive swelling on right upper for six months. child didn’t septic foci....

Antisense oligonucleotides (oligos) have been evaluated for treating prostate cancer in both in vivo and in vitro models. Although most oligos contain a single mRNA binding site, our laboratory evaluates bi-specific oligos directed towards two proteins. This study evaluates the growth inhibition in vitro of the LNCaP cell line employing monoand bispecific oligos directed against BCL-2 [the seco...

225 words Introduction – 370 words Discussion – 1521 words Abbreviations: PXR, pregnane X receptor; MRP, multidrug resistance-associated protein; RTPCR, reverse transcription-polymerase chain reaction; OATP, organic anion transporting peptide; 2-AAF, 2-acetylaminofluorene; BCRP, breast cancer resistance protein; CYP, cytochrome p450; ABC, ATP-binding cassette transporter; TCDD, 2,3,7,8-tetrachl...

Here we describe characterization of chicken neuronal Per-Arnt-Sim domain 3 (NPAS3) gene during embryogenesis including examinations of expression pattern and function of the gene. RTPCR assay showed that the primary tissue of expression for this gene is the central nervous system (CNS) while RNA in situ hybridization assay confirmed that NPAS3 was expressed in the ventricular zone of developin...

Results The i.v. and i.n. challenged mice showed significant DTH response. The CFU enumeration in eye did not show any bacilli initially by 30th day however tubercle bacilli could be recovered from the eyes of i.v. infected mice by 45 day at the time of death. Histopathology of these eyes showed inflammation with cluster of lymphocytes. The RTPCR for IS6110 gene from the eyes of i.n. infected a...

Methods We collected blood samples from a cohort of pediatric rheumatologic patients and scored them according to a qPCR based IFN gene signature assay. Expression of 6 type I IFN-related genes (IFI27, IFI44L, IFIT1, ISG15, RSAD2, SIGLEC1) was quantified by standard RTPCR techniques. An IFN score was calculated for each patient using the median fold change of gene expression related to a health...

This work presents a model for electrolyte-gated graphene field-effect transistors (EGFETs) that incorporates the effects of the double layer capacitance and the quantum capacitance of graphene. The model is validated through experimental graphene EGFETs, which were fabricated and measured to provide experimental data and extract graphene EGFET parameters such as mobility, minimum carrier conce...