PURPOSE To evaluate chondrocyte viability using confocal laser microscopy (CLM) following exposure to bipolar radiofrequency energy (bRFE) and to contrast CLM with standard light microscopy (LM) techniques. TYPE OF STUDY In vitro analysis using chondromalacic human cartilage. METHODS Twelve fresh chondral specimens were treated with the ArthroCare 2000 bRFE system (ArthroCare, Sunnyvale, CA...

BACKGROUND Systemic sclerosis or scleroderma is a rare collagen disease, characterized by insufficient angiogenesis. Few data are available about the morphologic and histochemical peculiarities of the skin in these patients with this condition. The purpose of the present work was to evaluate the histochemical aspects of sclerodermic skin, obtained through biopsy of the typical lesions from the ...

BACKGROUND The healing potential of articular cartilage in response to injury is poor, because articular cartilage lacks blood vessels or perichondrium. Costal cartilage is covered with a vascularized perichondrium, which is known to have chondrogenic potential. The purpose of this study was to determine whether costal cartilage can heal in response to mechanical injury. METHODS Sixty-five IC...

immobilization of catalysts on the surface of some inert supports makes therecovering step easier. because of the specific physicochemical properties, zeolites are good candidate as catalyst supports. in this study, zeolite x was synthesized by natural kaolin and tio2 was incorporated into zeolite phase by impregnation method. degradation of safranin orange, methylene blue and 2,4–dinitroanilin...

In the supporting information section of this paper, the structures of oltipraz and safranin in Figure S1 were incorrect. The corrected fi gure may be found at Copyright: © 2007 Kuntz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original au...

Various imaging techniques have been widely used to study intervertebral disc (IVD) biology, among which histological methods are most common as they are indispensable tools to retrieve structural information. Stainings using Alcian blue-fast red and safranin-O-fast green are popular but they have limitations in delineating the complex structures of the IVD comparing to their use for articular ...

The aim of the study was to evaluate the mast cell-blood vessel relationship using double staining CD34/AAS. Sections from 14 cases with lip tumors have been stained with Hematoxylin-Eosin. On additional sections from each case, we highlighted blood vessels by immunohistochemistry for CD34 antigen using the method LSAB2-HRP/DAB, followed by alcian blue-safranin stain for mast cells. We quantifi...

Soft agar culture of mononuclear cell fractions prepared from rat peripheral blood yielded numerous colonies consisting of mast cells. The mast cell nature of the cells was established by ultrastructural and histochemical analyses as well as by the demonstration the the colonies contained histamine and that the cells possessed receptors for the Fc component of IgE. Stringent criteria for the di...

OBJECTIVE To evaluate the effect of murine interleukin 17 (IL17) on cartilage catabolism and joint inflammation by direct intra-articular injection of the cytokine into murine knee joints. METHODS Knees of normal C57 Bl mice were injected once or repeatedly with recombinant IL17 or IL1beta. Inflammation was estimated by technetium-99m pertechnetate ((99)Tc) uptake and histological scoring of ...

OBJECTIVE This preliminary study aims to determine the differentially expressed proteins from chondrogenic differentiated multipotent stromal cells (cMSCs) in comparison to undifferentiated multipotent stromal cells (MSCs) and adult chondrocytes (ACs). METHODS ACs and bone marrow-derived MSCs were harvested from New Zealand White rabbits (n = 3). ACs and cMSCs were embedded in alginate and we...