background: formation of secondary structure such as dna hairpins or loops may influence molecular genetics methods and pcr based approaches necessary for genetic engineering, in addition to gene regulation. materials and methods: a polymerase chain reaction with splice overlap extension (soe-pcr) was used to create fully synthetic 1f5 chimeric anti-cd20 heavy- and light-chain genes. the chimer...

hairy root systems are formed by transforming plant tissues with the “natural genetic engineer” agrobacterium rhizogenes. in most plants such as cichorium intybus l., hairy root cultures have proven to be an efficient system for secondary metabolites production. the effect of zinc (znso4), a heavy metal, was investigated at different concentrations (0, 1, 5 and 10 mm) on some secondary metaboli...

fracture mapping of late permian-early triassic flat-lying sedimentary rocks in the sydney basin,new south wales, australia, shows that joints developed originally in extension were faulted in subsequentevents. joints with a regional distribution fall into two (early and late) formed groups. group i joints propagatedhorizontally and never interfered with each other. these joints were subsequent...

background & aim: sweet basil (ocimum basilicum), a plant that is extensively cultivated in some countries, is used to enhance the flavour of salads, sauces, pasta, confectioneries and other products as both a fresh and dried herb. the effect of foliar application of jasmonic acid and reduced irrigation on essential oil yield and chemical components of two iranian landrace of basil (ocimum ...

background & aim: medicinal plants are most valuable and useful sources as productive factors of drugs. production and extraction of secondary metabolites have a huge economical importance in recent years. carvone is a compound in bunium persicum fruit and shoot and, it is one of the wide range varieties of valuable and useful secondary metabolites. due to limited crop production, using app...

objective: secondary caries is a common problem in dental treatments and its occurrence is attributed to several factors. the present study aimed at assessing the prevalence of secondary caries in posterior teeth of patients presenting to the department of operative dentistry, shahid beheshti dental school.   methods: this descriptive study was conducted on 350 patients with 1,339 posterior tee...

abstract the aim of this study was the identify cyanobacterial flora of rice–fields in dargaz, to interaction between cyanobacteria by culturing in solid medium bg-110 in laboratory was investigated. nostoc piscinale and nostoc spongiaeforme were identified as strongest and weakness species, respectively, regarding secondary metabolits producing (allelochemicals).the algal pairs were classified...

fenugreek (trigonella foenum-graecum l.) has a long and respected history of medicinal uses in middle east and persian medicine. the hypocholesterolaemic and hypoglycaemic effects of fenugreek were attributed to its major steroidal sapogenin, diosgenin and its major alkaloid, trigonelline. the knowledge of diosgenin and trigonelline biosynthesis is derived from studies of cholesterol and nicoti...

purpose: to determine the incidence and risk factors associated with glaucoma following congenital cataract surgery (ccs) in children under age of 15 methods: this prospective cohort (since 2006) consisted of children less than 15 years of age who underwent cataract surgery with or without intraocular lens (iol) implantation. the role of the following factors on the development of glaucoma afte...

in this study, residual amount of pentachlorophenol (pcp) as the most important paper preservative, which is extremely hazardous pollutant, was determined in some tissue papers and napkins. twenty-five samples of two producing hygienic paper factories prepared from virgin and secondary pulp were analyzed for the presence of trace amount of pcp. the analytical procedure involved direct extractio...