The study of protein structures’ local conformations has a long history principally based on the analysis of the classical repetitive structures (i.e. α-helix and β-sheet), and also on the characterization of some particular structures in the coil state (e.g. turns). The secondary structures are interesting for describing the global protein fold but miss all the orientations of the connecting r...

background: formation of secondary structure such as dna hairpins or loops may influence molecular genetics methods and pcr based approaches necessary for genetic engineering, in addition to gene regulation. materials and methods: a polymerase chain reaction with splice overlap extension (soe-pcr) was used to create fully synthetic 1f5 chimeric anti-cd20 heavy- and light-chain genes. the chimer...