In connection with certain investigations being carried out in this laboratory, it was found necessary to work with sixto twelve-hour cultures of bacteria in fluid culture media. The apparatus described below was devised to obviate the need of either coming to the laboratory late in the evening to inoculate cultures for the following day's work or of wasting time waiting for cultures to grow ou...

The reasons for using sterilized pus in the place of autogenous or other vaccines are : 1. The great simplicity and cheapness. 2. The pus is truly autogenous, this is not so with vaccines prepared from organisms grown on artificial media; for the organism is changed by such growth. 3. Besides the bacteria, pus contains toxins, and antibodies both extremely useful for therapeutic inoculation. Th...

INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France; INRA, UR444 Laboratoire de génétique cellulaire, F-31320 CastanetTolosan, France; INRA, UMR1286; CNRS, UMR5226, Laboratoire PsyNuGen, F-33076 Bordeaux, France; INRA, UE967 Génétique expérimentale en productions animales, F-17700 Surgères, France; INRA, UAR2 Services déconcentrés d’appui à la recherche – Poit...

Fungi that occur inside asymptomatic plant tissues are known as fungal endophytes. Different genera of fungal entomopathogens have been reported as naturally occurring fungal endophytes, and it has been shown that it is possible to inoculate plants with fungal entomopathogens, making them endophytic. Their mode of action against insects appears to be due to antibiosis or feeding deterrence. Res...

The ever increasing movement of viruses around the world poses a major threat to plants growing in cultivated and natural ecosystems. Both generalist and specialist viruses move via trade in plants and plant products. Their potential to damage cultivated plants is well understood, but little attention has been given to the threat such viruses pose to plant biodiversity. To address this, we stud...

The estuarine copepod Acartia tonsa naturally carried diverse strains of bacteria on its body. The bacterial community composition (BCC) remained very conservative even when the copepod was fed different axenic algal species, indicating that the food per se did not much affect BCC associated with the copepod. In xenic algal treatments, however, copepod-associated BCC differed with each alga fed...

rpm). An inoculum of 5-8 % of the rusulting seed broth was then used to inoculate the fermentation flasks. The composition of the fermentation mediumwas as follows : sucrose 7 %, soybean meal 2.5 %, ammonium sulfate 0.3 %, pH 7.0, 25 ml of medium being used per 200ml flask. The fermentation was carried out on a rotary shaker at 200 rpm for about 10 days at 30°C. Feeding of S35-ammonium sulfate ...

Zygomycosis was produced experimentally in Asian water buffalo calves (Bubalus bubalis) by intratracheal inoculation of sporangiospores of Absidia corymbifera. Infected animals exhibited dullness, depression, partial anorexia, initial pyrexia, mucopurulent nasal and ocular discharge and coughing during the first week. There was no mortality at any stage of the experiment, which continued for 30...

BACKGROUND Severe early life stress (ELS) is associated with negative outcomes. It is not clear, however, what impact moderate ELS has. A growing stress inoculation literature suggests that moderate (vs. low or high) ELS is associated with diminished behavioral and physiological anxiety responses. At the same time, studies of trait anxiety suggest that moderate (vs. low) ELS is associated with ...