PURPOSE Report the characteristics of cryopreserved semen from a cohort of male cancer patients, attitudes towards cryopreservation and outcomes of semen samples based on a 12-year cryopreservation program. MATERIAL AND METHODS Data from 98 male cancer patients whose sperm samples were banked were evaluated. Demographic parameters, semen characteristics, destination of sperm banked samples an...

The objective of this study was to test the effect of four different diluents on the motility of goat semen stored at room and refrigerator temperatures. To achieve this objective, four West African dwarf bucks aged 10-12 months were ejaculated weekly for five weeks using the electro-ejaculator method. The ejaculated semen were extended using four diluents namely sodium citrate (T1), sodium bic...

The aim of the experiment was to determine the effect of potential antioxidants (adenosine, L-cysteine hydrochloride, ascorbic acid, magnesium fumarate and prolactin) supplementing the Biosolwens extender on semen survival time and sperm chromatin structure. The semen motility was examined every day and the susceptibility of sperm chromatin to denaturation was evaluated on collection day and da...

The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4°C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred an...

The present study was aimed to see the effect of butylated hydroxytoluene (BHT) as semen additives during cryopreservation of Hariana Bull semen. Twenty four ejaculates from two Hariana bulls extended with glycerolated egg yolk tris (GEYT). Extended samples were split into three aliquots. One aliquot was kept as control while other two aliquots were supplemented with 0.5 mM (T1) and 1.0 mM (T2)...

Modern livestock breeding is basically dependent on the proper use of semen for artificial insemination of females and of other reproductive biotechnologies such as the production of embryos in vitro for embryo transfer. Both these techniques have made possible not only the wide dissemination of genetic material onto breeding populations but also enhanced the selection of best sires, owing to t...

Our recent studies suggested that the freezability of carp semen is related to seminal plasma protein profiles. Here, we aimed to compare the spermatozoa proteomes of good (GF) and poor (PF) freezability semen of carp. To achieve this, we used two-dimensional difference in gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. The semen was classified as GF or PF based on sperm motili...

The preservation of spermatozoa is an important tool used in conservation programs to increase the genetic diversity of threatened and endangered species. Although routinely used to manage conservation programs for higher vertebrates, there have been limited attempts to establish reproductive assistance programs for tortoises. The purpose of this study was to develop a model for collecting and ...

Exosomes are important vehicles of intercellular communication that shape host responses to physiologic, tumorigenic, and pathogenic conditions. The composition and function of exosomes are dynamic and depends on the state and condition of the cellular source. In prior work, we found that semen exosomes (SE) from healthy donors who do not use illicit drugs potently inhibit HIV-1. Following seme...

The current population of wisents (Bison bonasus, Linnaeus 1758) originates from only 12 individuals that survived World War II in zoos and private enclosures. For better preservation genetic variability, research is being conducted to introduce assisted reproductive techniques this species. In article, we describe the state knowledge prospects for further development field. article divided int...