Evidence supports the involvement of nitric oxide (NO) in a Varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, Low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established t...

Seminal plasma is a promising biological fluid to use for noninvasive clinical diagnostics of male reproductive system disorders. To verify a list of prospective male infertility biomarkers, we developed a multiplex selected reaction monitoring assay and measured the relative abundance of 31 proteins in 30 seminal plasma samples from normal, nonobstructive azoospermia and post-vasectomy individ...

background: the trace element copper has been identified as a highly toxic element for sperm. it is known to affect sperm motility in humans and experimental implantation of copper in the epididymis vas deferens and scrotum of mammals has been demonstrated to affect fertility detrimentally. objective: sperm concentration motility vitality and morphology are parameters used to evaluate potential...

INTRODUCTION The exact causes of the decline in semen quality are not yet known, environmental factors have been considered to play an important role. Lead (Pb) and Cadmium (Cd) are two of the well-known reproductive toxicants to which humans are exposed occupationally and environmentally and can lead to negative effects on the testicular functions. The aim of this study was to evaluate lead an...

background: there is growing evidence that damage to spermatozoa by reactive oxygen species (ros) play a key role in male infertility. objective: the aim of the present study was to assess the antioxidant status of seminal plasma by measuring total antioxidant capacity (tac) and activities of catalase and superoxide dismutase (sod) in men with asthenozoospermia, asthenoteratozoospermia and olig...

Two experiments were designed to examine the effects of crude seminal plasma (CSP) exposure ofejaculated and epididymal spermatozoa before freezing. Experiment 1, two consecutive ejaculates werecollected (n=4) within 5 min, the second one was carried out in a tube containing Fiser extender (coatedspermatozoa); by centrifugation, seminal plasma was removed from coated ejaculates. The pellets wer...

background: the lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. the purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (mda) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. in addition, correlation of lipid peroxidation with mot...

background: it has been proposed that oxidative stress plays an important role in male infertility. the aims of this study were to compare seminal plasma levels of 15-f2t-isoprostane (8-iso-pgf2?), malondialdehyde (mda), and total (sum of free and bound) homocysteine (thcy) in normozoospermic vs. asthenozoospermic men, and to examine the relationships between thcy and lipid peroxidation product...