Temperature-induced changes in protein intrinsic fluorescence of native, delipidated and deionized purple membranes are investigated. It is found that the removal of cations most strongly affects the protein and its thermal stability. The denaturation of dei-BR completes at 70 degrees C, while delipidated and native BR still maintain their native structure at this temperature. Both the quantum ...

As the field nephrology is rapidly advancing, technological changes are changing the face of learning. Understanding how the current generation of “digital natives” learn and understand topics is vastly important. The current students and residents were born when computers and electronic gadgets were considered a part and parcel of their learning structures. The primary focus of this review is ...

The role of direct methods in macromolecular crystallography is discussed. The common belief that such methods will still remain marginal is rejected. Different sectors are analyzed. A direct procedure for phasing reflections when diffraction data of one isomorphous derivative are available is briefly described. The applications to experimental data of some test structures succeeded, and sugges...

Topically applied camphor elicits a sensation of cool, but nothing is known about how it affects cold temperature sensing. We found that camphor sensitizes a subpopulation of menthol-sensitive native cutaneous nociceptors in the mouse to cold, but desensitizes and partially blocks heterologously expressed TRPM8 (transient receptor potential cation channel subfamily M member 8). In contrast, cam...

Smirnovia iranica, a native valuable woody species from Fabaceae, is an adaptable plant of central sandy areas of Iran. Changes in chlorophyll fluorescence and photosynthetic pigments characteristics were analyzed in the course of phenological stages including vegetative, flowering, seed ripening, and seed falling stages, respectively (VS, FS, SRS, and SFS). The results obtained from analysis o...

The red fluorescent protein, DsRed, recently cloned from coral Discosoma sp. has one of the longest fluorescence waves and one of the most complex absorbance spectra among the family of fluorescent proteins. In this work we found that with time DsRed fluorescence decreases under mildly acidic conditions (pH 4.0-4.8) in a pH-dependent manner, and this fluorescence inactivation could be partially...